Aims: Uveal melanomas with or without monosomy 3 have different metastatic potentials. Therefore, it would be of great experimental importance to obtain cell lines of both groups – established and characterized under the same conditions. Methods: Long-term culture of biopsies derived from untreated primary uveal melanomas was performed, and the status of chromosome 3 in the tumour was determined. Characterization by immunocytochemistry and in vitro assays for cell migration, vasculogenic cord formation and proliferation were performed. Results: Establishment of cell lines succeeded in 6 out of 128 uveal melanomas. No histopathological feature was predictive for cultivation success. Two out of 4 cell lines derived from tumours with monosomy 3 were invasive in vitro. Both cell lines with disomy 3 showed higher proliferation levels (p = 0.002). Conclusions: The in vitro tendency for invasion did not correlate with the proliferative behaviour of uveal melanoma cell lines, but was partly associated with monosomy of chromosome 3.

Anastassiou G, Tschentscher F, Zeschnigk M: Prognostically relevant markers of malignant melanoma of the uvea. Ophthalmologe 2002;99:327–332.
Prescher G, Bornfeld N, Hirche H, Horsthemke B, Jockel KH, Becher R: Prognostic implications of monosomy 3 in uveal melanoma. Lancet 1996;347:1222–1225.
Tschentscher F, Husing J, Holter T, Kruse E, Dresen IG, Jockel KH, Anastassiou G, Schilling H, Bornfeld N, Horsthemke B, Lohmann DR, Zeschnigk M: Tumor classification based on gene expression profiling shows that uveal melanomas with and without monosomy 3 represent two distinct entities. Cancer Res 2003;63:2578–2584.
Albert DM, Ruzzo MA, McLaughlin MA, Robinson NL, Craft JL, Epstein J: Establishment of cell lines of uveal melanoma: methodology and characteristics. Invest Ophthalmol Vis Sci 1984;25:1284–1299.
Aubert C, Rouge F, Reillaudou M, Metge P: Establishment and characterization of human ocular melanoma cell lines. Int J Cancer 1993;54:784–792.
De Waard-Siebinga I, Blom DJ, Griffioen M, Schrier PI, Hoogendoorn E, Beverstock G, Danen EH, Jager MJ: Establishment and characterization of an uveal-melanoma cell line. Int J Cancer 1995;62:155–161.
Luyten GP, Naus NC, Mooy CM, Hagemeijer A, Kan Mitchell J, Van Drunen E, Vuzevski V, De Jong PT, Luider TM: Establishment and characterization of primary and metastatic uveal melanoma cell lines. Int J Cancer 1996;66:380–387.
Ksander BR, Rubsamen PE, Olsen KR, Cousins SW, Streilein JW: Studies of tumor-infiltrating lymphocytes from a human choroidal melanoma. Invest Ophthalmol Vis Sci 1991;32:3198–3208.
Maniotis AJ, Chen X, Garcia C, De Christopher PJ, Wu D, Pe’er J, Folberg R: Control of melanoma morphogenesis, endothelial survival, and perfusion by extracellular matrix. Lab Invest 2002;82:1031–1043.
Maniotis AJ, Folberg R, Hess A, Seftor EA, Gardner LM, Pe’er J, Trent JM, Meltzer PS, Hendrix MJ: Vascular channel formation by human melanoma cells in vivo and in vitro: vasculogenic mimicry. Am J Pathol 1999;155:739–752.
Nareyeck G, Zeschnigk M, Prescher G, Lohmann DR, Anastassiou G: Establishment of two uveal melanoma cell lines derived from tumors with loss of one chromosome 3. Exp Eye Res 2006;83:858–864.
Hu DN, McCormick SA, Ritch R, Pelton-Henrion K: Studies of human uveal melanocytes in vitro: isolation, purification and cultivation of human uveal melanocytes. Invest Ophthalmol Vis Sci 1993;34:2210–2219.
Nareyeck G, Wuestemeyer H, von der Haar D, Anastassiou G: Establishment of two cell lines derived from conjunctival melanomas. Exp Eye Res 2005;81:361–362.
Tschentscher F, Prescher G, Zeschnigk M, Horsthemke B, Lohmann DR: Identification of chromosomes 3, 6 and 8 aberrations in uveal melanoma by microsatellite analysis in comparison to comparative genomic hybridization. Cancer Genet Cytogenet 2000;122:13–17.
Nareyeck G, Rauterberg J, von der Haar D, Eble JA, Anastassiou G: Cell adhesion experiments with cell lines derived from uveal melanomas with monosomy 3. Invest Ophthalmol Vis Sci 2005;46:E-abstract 3376.
Guan J, Di Biase S, Iliakis G: The catalytic subunit DNA-dependent protein kinase (DNA-PKcs) facilitates recovery from radiation-induced inhibition of DNA replication. Nucleic Acids Res 2000;28:1183–1192.
Coupland SE, Sidiki S, Clark BJ, McClaren K, Kyle P, Lee WR: Metastatic choroidal melanoma to the contralateral orbit 40 years after enucleation. Arch Ophthalmol 1996;114:751–756.
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