Purpose: To determine the potential of culture in vitro to alter the human leukocyte antigen (HLA) molecules and costimulatory molecules on human retinal pigment epithelium (RPE). Methods: Pure RPE were isolated and cultured. Two sets of RPE (normal and activated) were used. Activated RPE were obtained by incubating primary cultures of RPE with recombinant human interferon-γ. The expression of HLA molecules and costimulatory molecules on human RPE at different passages after culture in vitro were analyzed quantitatively by flow cytometry. Results: In the process of routine culture on culture flask, the duration of culture in vitro was significantly correlated with the expression of HLA-ABC molecules on the normal RPE and the activated RPE (r = –0.893, p < 0.001 and r = –0.964, p < 0.001 respectively), HLA-DR molecule on the activated RPE (r = –0.901, p < 0.001) and intercellular adhesion molecule-1 on the normal RPE (r = 0.961, p < 0.001). There were no correlations between the duration of culture in vitro andthe expression of HLA-DR molecule on the normal RPE, intercellular adhesion molecule-1 on the activated RPE, B7-1 and B7-2 molecules on the normal RPE and the activated RPE. Conclusions: The chronic rejection is the major immunological rejection following RPE allogeneic graft. Culture in vitro modulation of HLA molecules and costimulatory molecules on RPE may increase lymphocyte infiltration and activation and promote the chronic rejection following allograft RPE transplantation.

1.
Radtke ND, Aramant RB, Seiler MJ, Petry HM, Pidwell D: Vision change after sheet transplant of fetal retina with retinal pigment epithelium to a patient with retinitis pigmentosa. Arch Ophthalmol 2004;122:1159–1165.
2.
Enzmann V, Faude F, Wiedemann P, Kohen L: Immunological problems of transplantation into the subretinal space. Acta Anat (Basel) 1998;162:178–183.
3.
Devine L, Lightman SL, Greenwood J: Role of LFA-1, ICAM-1, VLA-4 and VCAM-1 in lymphocyte migration across retinal pigment epithelial monolayers in vitro. Immunology 1996;88:456–462.
4.
McKay BS, Burke JM: Separation of phenotypically distinct subpopulations of cultured human retinal pigment epithelial cells. Exp Cell Res 1994;213:85–92.
5.
Devine L, Lightman S, Greenwood J: Lymphocyte migration across the anterior and posterior blood-retinal barrier in vitro. Cell Immunol 1996;168:267–275.
6.
Farrokh-Siar L, Rezai KA, Palmer EM, Patel SC, Ernest TJ, van Seventer GA: Cytokine modulation of costimulatory molecules on human fetal retinal pigment epithelial cells. Curr Eye Res 2001;23:285–290.
7.
Nagineni CN, Kutty RK, Detrick B, Hooks JJ: Inflammatory cytokines induce intercellular adhesion molecule-1 (ICAM-1) mRNA synthesis and protein secretion by human retinal pigment epithelial cell cultures. Cytokine 1996;8:622–630.
8.
Rezai KA, Semnani RT, Patel SC, Ernest JT, van Seventer GA: The immunogenic potential of human fetal retinal pigment epithelium and its relation to transplantation. Invest Ophthalmol Vis Sci 1997;38:2662–2671.
9.
Enzmann V, Hollborn M, Wiedemann P, Kohen L: Molecular and cellular evidence for T-cell stimulation by allogeneic retinal pigment epithelium cells in vitro. Graefes Arch Clin Exp Ophthalmol 2001;239:445–451.
10.
Farrokh-Siar L, Rezai KA, Semnani RT, Patel SC, Ernest JT, van Seventer GA: Human fetal retinal pigment epithelium suppresses the activation of CD4+ and CD8+ T-cells. Graefes Arch Clin Exp Ophthalmol 1999;237:934–939.
11.
Holtkamp GM, Kijlstra A, Peek R, de Vos AF: Retinal pigment epithelium-immune system interactions: cytokine production and cytokine-induced changes. Prog Retin Eye Res 2001;20:29–48.
12.
Ghosh F, Larsson J, Wilke K: MHC expression in fragment and full-thickness allogeneic embryonic retinal transplants. Graefes Arch Clin Exp Ophthalmol 2000;238:589–598.
13.
Grisanti S, Guidry C: Transdifferentiation of retinal pigment epithelial cells from epithelial to mesenchymal phenotype. Invest Ophthalmol Vis Sci 1995;36:391–405.
14.
Engelmann K, Valtink M: RPE cell cultivation. Graefes Arch Clin Exp Ophthalmol 2004;242:65–67.
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