Purpose: To determine the intraocular levels of methotrexate in low-dose treatment of noninfectious uveitis. Methods: One day after oral administration, the methotrexate level was measured in the aqueous humor and serum of a patient with noninfectious uveitis, who underwent cataract surgery. A fluorescence polarization immunoassay was used for determination. Results: After oral administration, methotrexate was only measurable in aqueous humor but not in serum. Conclusion: In uveitis, orally administered low-dose methotrexate reaches detectable levels in aqueous humor, even in the absence of detectable levels in serum.

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