A new tumor cell line (SUIT-4) derived from ascites of a patient with carcinoma of the pancreas has been established in tissue culture and in nude mice, and maintained for over 7 years. In tissue culture, the cells grew as a confluent monolayer with piling up of cells in some areas. The population doubling time during the exponential phase of the cell growth was 43.9 h in vitro. Chromosome count ranged from 63 to 68 with a modal number of 67. Subcutaneous injection of cultured cells into the flanks of nude mice resulted in tumor formation with a doubling time of 88.8 h. Histopathologically, xenografts in nude mice were moderately differentiated tubular adenocarcinoma, and the tumor cells showed spontaneous metastasis to the regional lymph nodes in 6 of 21 nude mice and to the lung in 4 of 21. Transmission electron microphotographs confirmed the ductal cell origin of the carcinoma and revealed that the cells had abundant mitochondria and lysosomes. SUIT-4 cells released carcinoembryonic antigen (3.08 × 102 ng/1 × 106 cells/24 h) and carbohydrate antigen 19-9 (4.75 × 104 U/1 × 106 cells/24 h) during exponential cell growth in vitro. Reverse transcriptase-polymerase chain reaction studies revealed that SUIT-4 cells expressed matrix metalloproteinases 1, 3, 7, 10 and 14.

Health and Welfare Statistics Association: Death. J Health Welf Stat 2000;47:48–60.
Saito Y: Investigation of pancreatic carcinoma registration research in Japan. Suizo 1998;13:63–91.
Kobari M, Matsuno M: Stage classification for pancreatic cancer: Its difference between Japan and UICC (in Japanese). Nippon Shokakibyo Gakkai Zasshi 1997;94:1–7.
Iwamura T, Katsuki T, Ide K: Establishment and characterization of a human pancreatic cancer cell line (SUIT-2) producing carcinoembryonic antigen and carbohydrate antigen 19-9. Jpn J Cancer Res 1987;78:54–62.
Iwamura T, Katsuki T: Kinetics of carcinoembryonic antigen and carbohydrate antigen 19-9 production in a human pancreatic cancer cell line (SUIT-2). Gastroenterol Jpn 1987;22:640–646.
Iwamura T, Taniguchi S, Kitamura N, Yamanari H, Kojima A, Hidaka K, Setoguchi T, Katsuki T: Correlation between CA19-9 production in vitro and histological grades of differentiation in vivo in clones isolated from a human pancreatic cancer cell line (SUIT-2). J Gastroenterol Hepatol 1992;7:512–519.
Iwamura T, Caffrey TC, Kitamura N, Yamanari H, Setoguchi T, Hollingsworth MA: P-selectin expression in a metastatic pancreatic tumor cell line (SUIT-2). Cancer Res 1997;57:1206–1212.
Taniguchi S, Iwamura T, Katsuki T: Correlation between spontaneous metastatic potential and type I collagenolytic activity in a human pancreatic cancer cell line (SUIT-2) and sublines. Clin Exp Metastasis 1992;10:259–266.
Taniguchi S, Iwamura T, Kitamura N, Yamanari H, Kojima A, Hidaka K, Seguchi K, Setoguchi T: Protein-free culture of the human pancreatic cancer cell line, SUIT-2. Hum Cell 1994;7:207–214.
Yamanari H, Suganuma T, Iwamura T, Kitamura N, Taniguchi S, Setoguchi T: Extracellular matrix components regulating glandular differentiation and the formation of basal lamina of a human pancreatic cancer cell line in vitro. Exp Cell Res 1994;211:175–182.
Dobrynin YV: Establishment and characteristics of cell strains from some epithelial tumors of human origin. J Natl Cancer Inst 1963;31:1173–1195.
Iwamura T, Hollingsworth MA: Pancreatic tumors; in Masters JRW, Palsson B (eds): Cancer Cell Lines. New York, Kluwer Academic, 1998, vol 1, part I: Human Cell Culture, pp 107–122.
Kyriazis AP, Kyriazis AA, Scarpelli DG, Fogh J, Rao MS, Lepera R: Human pancreatic adenocarcinoma line Capan-1 in tissue culture and the nude mouse: Morphologic, biologic, and biochemical characteristics. Am J Pathol 1982;106:250–260.
Chen J: Establishment and studies of two human pancreatic carcinoma in vivo lines by transplanting the tumor into nude mice (in Chinese). Zhonghua Bing Li Xue Za Zhi 1989;18:197–200.
Grunewald K, Lyons J, Frohlich A, Feichtinger H, Weger RA, Schwab G, Janssen JW, Bartram CR: High frequency of Ki-ras codon 12 mutations in pancreatic adenocarcinomas. Int J Cancer 1989;43:1037–1041.
Yasuda D, Iguchi H, Ikeda Y, Nishimura S, Steeg PS, Misawa T, Nawata H, Kono A: Possible association of Nm23 gene expression and Ki-ras point mutations with metastatic potential in human pancreatic cancer-derived cell lines. Int J Oncol 1993;3:641–644.
Almoguera C, Shibata D, Forrester K, Martin J, Arnheim N, Perucho M: Most human carcinomas of the exocrine pancreas contain mutant c-K-ras genes. Cell 1988;53:549–554.
Motojima K, Urano T, Nagata Y, Shiku H, Tsunoda T, Kanematsu T: Mutations in the Kirsten-ras oncogene are common but lack correlation with prognosis and tumor stage in human pancreatic carcinoma. Am J Gastroenterol 1991;86:1784–1788.
Gress TM, Muller-Pillasch F, Lerch MM, Friess H, Buchler M, Adler G: Expression and in-situ localization of genes coding for extracellular matrix proteins and extracellular matrix degrading proteases in pancreatic cancer. Int J Cancer 1995;62:407–413.
Sato H, Takino T, Okada Y, Cao J, Shinagawa A, Yamamoto E, Seiki M: A matrix metalloproteinase expressed on the surface of invasive tumour cells. Nature 1994;370:61–65.
Stetler-Stevenson WG, Krutzsch HC, Liotta LA: Tissue inhibitor of metalloproteinase (TIMP-2). A new member of the metalloproteinase inhibitor family. J Biol Chem 1989;264:17374–17378.
Urbanski SJ, Edwards DR, Maitland A, Leco KJ, Watson A, Kossakowska AE: Expression of metalloproteinases and their inhibitors in primary pulmonary carcinomas. Br J Cancer 1992;66:1188–1194.
Kitamura N, Iwamura T, Taniguchi S, Yamanari H, Kawano K, Hollingsworth MA, Setoguchi T: High collagenolytic activity in spontaneously highly metastatic variants derived from a human pancreatic cancer cell line (SUIT-2) in nude mice. Clin Exp Metastasis 2000;18:561–571.
Duivenvoorden WC, Hirte HW, Singh G: Transforming growth factor beta1 acts as an inducer of matrix metalloproteinase expression and activity in human bone-metastasizing cancer cells. Clin Exp Metastasis 1999;17:27–34.
Kaku M, Nishiyama T, Yagawa K, Abe M: Establishment of a carcinoembryonic antigen-producing cell line from human pancreatic carcinoma. Gann 1980;71:596–601.
Morgan RT, Woods LK, Moore GE, Quinn LA, McGavran L, Gordon SG: Human cell line (COLO 357) of metastatic pancreatic adenocarcinoma. Int J Cancer 1980;25:591–598.
Dexter DL, Matook GM, Meitner PA, Bogaars HA, Jolly GA, Turner MD, Calabresi P: Establishment and characterization of two human pancreatic cancer cell lines tumorigenic in athymic mice. Cancer Res 1982;42:2705–2714.
Yamaguchi N, Uozumi G, Ikeuchi H, Morioka H, Machino M, Kawai K: Establishment and characterization of a carcinoembryonic antigen producing cell line derived from human pancreatic exocrine cancer. Gastroenterol Jpn 1983;18:585–592.
Okabe T, Yamaguchi N, Ohsawa N: Establishment and characterization of a carcinoembryonic antigen (CEA)-producing cell line from a human carcinoma of the exocrine pancreas. Cancer 1983;51:662–668.
Kyriazis AP, McCombs WB 3rd, Sandberg AA, Kyriazis AA, Sloane NH, Lepera R: Establishment and characterization of human pancreatic adenocarcinoma cell line SW-1990 in tissue culture and the nude mouse. Cancer Res 1983;43:4393–4401.
Kobari M, Hisano H, Matsuno S, Sato T, Kan M, Tachibana T: Establishment of six human pancreatic cancer cell lines and their sensitivities to anti-tumor drugs. Tohoku J Exp Med 1986;150:231–248.
Drucker BJ, Marincola FM, Siao DY, Donlon TA, Bangs CD, Holder WD Jr: A new human pancreatic carcinoma cell line developed for adoptive immunotherapy studies with lymphokine-activated killer cells in nude mice. In Vitro Cell Dev Biol 1988;24:1179–1187.
Ikeda Y, Ezaki M, Hayashi I, Yasuda D, Nakayama K, Kono A: Establishment and characterization of human pancreatic cancer cell lines in tissue culture and in nude mice. Jpn J Cancer Res 1990;81:987–993.
Schoumacher RA, Ram J, Iannuzzi MC, Bradbury NA, Wallace RW, Hon CT, Kelly DR, Schmid SM, Gelder FB, Rado TA, et al: A cystic fibrosis pancreatic adenocarcinoma cell line. Proc Natl Acad Sci USA 1990;87:4012–4016.
Kyriazis AA, Kyriazis AP, Sternberg CN, Sloane NH, Loveless JD: Morphological, biological, biochemical, and karyotypic characteristics of human pancreatic ductal adenocarcinoma Capan-2 in tissue culture and the nude mouse. Cancer Res 1986;46:5810–5815.
Fujii Y, Sekiguchi M, Shiroko Y, Shimizu H, Sugawara I, Hasumi K, Eriguchi M, Ikeuchi T, Uchida H: Establishment and characterization of human pancreatic adenocarcinoma cell line SOJ producing carcinoembryonic antigen and carbohydrate antigen 19-9 (in Japanese). Hum Cell 1990;3:31–36.
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