... and effector mechanisms in the kidney and brain, including the mineralocorticoid receptor, the serum- and glucocorticoid-induced kinase SGK1, the ubiquitin ligase NEDD4-2, and the epithelial sodium channel ENaC. The latter also mediates the gustatory salt sensing in the tongue, which is required...

...; Anselm A. Zdebik Background/Aims: Mutations in the inwardly-rectifying K + -channel KCNJ10/Kir4.1 cause autosomal recessive EAST syndrome (epilepsy, ataxia, sensorineural deafness and tubulopathy). KCNJ10 is expressed in the distal convoluted tubule of the kidney, stria vascularis of the inner ear...

... that pericytes can constrict/dilate descending vasa recta when vasoactive substances are present. We describe a live kidney slice model in which pericyte-mediated vasa recta constriction/dilation can be visualized in situ. Methods: Confocal microscopy was used to image calcein, propidium iodide and Hoechst...

... cause an autosomal recessive disorder characterized by epilepsy, ataxia, sensorineural deafness and tubulopathy (EAST syndrome). KCNJ10 is expressed in the kidney distal convoluted tubule, cochlear stria vascularis and brain glial cells. Patients clinically diagnosed with EAST syndrome were genotyped...

...G. Jacquillet; I. Rubera; R.J. Unwin The maintenance of sodium (Na + ) homeostasis is an essential function of the kidney. It is achieved by a variety of transport processes localized all along the highly specialised segments of the nephron. Impairment of these transport mechanisms, and thereby Na...

... was to re-investigate basolateral innervation of rat renal collecting duct epithelial cells by sympathetic nerves in the context of recent data on the effects of ATP on this nephron segment. Methods: Kidney sections were processed for electron immunocytochemistry, using tyrosine hydroxylase rabbit...

...Carolyn M. Feltes; Jennifer Van Eyk; Hamid Rabb Acute kidney injury (AKI) contributes significantly to morbidity and mortality in both adults and children. While clinical data suggest that AKI contributes to and exacerbates multiorgan failure, the physiologic and molecular mechanisms responsible...

... for cell transplantation after ischemic insult should be short. Conclusions: This is the first report of intra-arterial autologous transplantation of MSC in the kidney, resulting in a successful engraftment into tubular and glomerular structures. The results strongly suggest that the optimal time window...

...: To determine ANG II effects on renal DA metabolism, we evaluated 3 H-DA uptake in vitro in kidney tissue samples from Sprague-Dawley rats. Results: The results indicate that ANG II decreased DA uptake at 30 min, in a concentration-response fashion, in the external and juxtamedullar cortex. DA uptake...

..., Steinhausen et al. [ 3 ] utilized intravital videomicroscopy to demonstrate that peritubular blood flow on the cortical surface of the kidney was diminished following an ischemic insult. Recently, Yamamoto et al. [ 4 ] utilized a small pencil-lens videomicroscope to advance these findings. They applied...

...Isabelle Guinobert; Mélanie Viltard; David Piquemal; Jean-Marc Elalouf; Jacques Marti; Martine Lelièvre-Pégorier Background: The kidney development involves a wide variety of developmental processes requiring a lot of genes expressed in a sequential manner. The aim of the present study...

... Kidney Diabetes Streptozotocin Total RNA was extracted by the modified method of Chomczynski and Sacchi [ 14 ]. Slices of kidney cortex were homogenized in 4 M guanidium thiocyanate/0.75 M sodium citrate/0.1 M   p -mercaptoethanol/10% sarcosyl. RNA was extracted with phenol/sodium acetate...

... that activating or inhibitory phosphorylation of neuronal and endothelial nitric oxide synthase (nNOS, eNOS) regulates renal NO production in response to altered dietary salt. Methods: Sprague-Dawley rats were fed low, normal or high salt diets for 12 h or 2 weeks, and kidney NOS phosphorylation was analyzed...

... and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements. Apoptosis Zinc Kidney Cadmium intoxication The kidney is one of the main organs involved in trace element...

..., quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements. Streptozotocin Kidney Renalvascular wall thickening Diabetic glomerulopathy...

...Volker Vallon Gene-targeted mice provide a powerful approach to study the physiological and pathophysiological role of a given protein in kidney function and can give insights on the functional importance of these proteins under in vivo conditions as well as on the potential compensating mechanisms...

...Kenichi Ishibashi; Toshiyuki Matsuzaki; Kuniaki Takata; Masashi Imai Aim: To clone new phosphate transporters from the kidney. Methods: EST bank was screened for new members of Na-dependent inorganic phosphate (P i ) co-transporter. To obtain a full-length clone, a rat kidney cDNA library...

... or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements. Gravity Kidney Microalbuminuria Physiological changes occur in man during space...