Reperfusion injuries after organ transplantation affect graft function and influence long-term graft survival. As hypothermic storage, which minimizes the extent of unspecific tissue injury after ischemia and reperfusion, is significantly influenced by the composition of preservation solutions, strategies to optimize the different components may lead to longer graft survival. In the present study the effects of the preservation solution B2 on early renal function and histopathological changes were compared to histidine-tryptophan-ketoglutarate solution (HTK, Bretschneider) in a model of isolated blood-perfused porcine kidneys. B2-preserved kidneys displayed a lower renal resistance and significantly better creatinine clearance as compared to HTK. Mean differences were also found for filtration fraction and sodium fraction reabsorption. The functional data were also related to histopathological changes. Together, these data indicate that the recently developed preservation solution B2 offers new principles of preservation and is a useful preservation solution for experimental isolated perfused kidney models. B2 may also be an interesting model for optimizing preservation within other organ perfusion models.

1.
Gridelli B, Remuzzi G: Strategies for making more organs available for transplantation. N Engl J Med 2000;343:404–410.
2.
Suthanthiran M, Strom TB: Renal transplantation. N Engl J Med 1994;331:365–376.
3.
Savioz D, Jeanjacquot A, Savioz M, Ludwig C, Chilcott M, Bolle JF, Graf JD, Nastasi A, Morel P: Optimization of the kinetics of cooling of kidneys: A pig model. Eur Surg Res 1999;31:3–8.
4.
Satoh S, Stowe NT, Inman SR, Sankari BR, Magnusson MO, Novick AC: Renal vascular response to vasodilators following warm ischemia and cold storage preservation in dog kidneys. J Urol 1993;149:186–189.
5.
Fahy GM, Ali SE: Cryopreservation of the mammalian kidney. II. Demonstration of immediate ex vivo function after introduction and removal of 7.5 M cryoprotectant. Cryobiology 1997;35:114–131.
6.
Savioz D, Bolle JF, Graf JD, Jeanjacquot A, Savioz M, Dietler G, Favre H, Leski M, Morel D, Morel P: Kinetics of cellular viability in warm versus cold ischemia conditions of kidney preservation. A biometric study. Transplantation 1996;62:414–417.
7.
Cadrobbi R, Rigotti P, Baldan N, Toffano M, Corazza A, Scarpa M, Rigo A, Ancona E: Assessment of pretransplantation warm ischemia time by phosphorus-31 magnetic resonance spectroscopy in pig kidneys. Transplant Proc 1997;29:3415–3416.
8.
Bretan PN Jr, Lobo E, Chang JA, Dumitrescu O, Miller B, Yen TS: Assessment of preservation-induced reperfusion injury via intraoperative renal transplant blood flow and endothelin concentration studies. J Urol 1997;158:714–718.
9.
Kehrer G, Blech M, Kallerhoff M, Kleinert H, Langheinrich M, Bretschneider HJ: Glucose content and efficiency of glycolysis in protected ischemic kidneys of different species. J Invest Surg 1990;3:147–168.
10.
Hauet T, Mothes D, Goujon JM, Caritez JC, Carretier M, Eugene M: Evaluation of injury preservation in pig kidney cold storage by proton nuclear magnetic resonance spectroscopy of urine. J Urol 1997;157:1155–1160.
11.
Hauet T, Baumert H, Amor IB, Goujon JM, Gibelin H, Godart C, Vandewalle A, Carretier M, Eugene M: Protection of autotransplanted pig kidneys from ischemia-reperfusion injury by polyethylene glycol. Transplantation 2000;70:1569–1575.
12.
Goujon JM, Vandewalle A, Baumert H, Carretier M, Hauet T: Influence of cold-storage conditions on renal function of autotransplanted large pig kidneys. Kidney Int 2000;58:838–850.
13.
Pacholczyk M, Chmura A, Adadynski L, Szostek M, Michalak G, Ostrowski K, Walaszewski J, Rowinski W: In situ perfusion and UW solution used for storage did not decrease the incidence of ATN in kidneys harvested from hemodynamically unstable donors. Transpl Int 1994;7:S476–S478.
14.
von Baeyer H, Stahl K, Hausler M, Meissler M, Unger V, Frank J, Grosse-Siestrup C, Kaczmarczyk G, Affeld K, Flaig HJ, Steinbach B: A new method of ex vivo whole blood perfusion of isolated mammalian organs, exemplified by the kidney of swine (in German). Biomed Tech (Berl) 1997;42:61–68.
15.
Holscher M, Groenewoud AF: Current status of the HTK solution of Bretschneider in organ preservation. Transplant Proc 1991;23:2334–2337.
16.
Morpurgo E, Rigotti P, Capalbo M, Baldan N, Pittoni G, Valente ML, Bindoli A, Ancona E: Transplantation of warm ischemia damaged kidneys: An experimental study in pigs. Ren Fail 1993;15:581–585.
17.
Grosse-Siestrup C, Fehrenberg C, von Baeyer H, Groneberg DA: Multiple-organ harvesting for models of isolated hemoperfused organs of slaughtered pigs. ALTEX 2002;19:9–13.
18.
Grosse-Siestrup C, Unger V, Fehrenberg C, von Baeyer H, Fischer A, Schaper F, Groneberg DA: A model of isolated autologously hemoperfused porcine slaughterhouse kidneys. Nephron 2002;92:414–421.
19.
Groneberg DA, Hartmann P, Dinh QT, Fischer A: Expression and distribution of vasoactive intestinal polypeptide receptor VPAC(2) mRNA in human airways. Lab Invest 2001;81:749–755.
20.
Groneberg DA, Eynott PR, Oates T, Lim S, Wu R, Carlstedt I, Nicholson AG, Chung KF: Expression of MUC5AC and MUC5B mucins in normal and cystic fibrosis lung. Respir Med 2002;96:81–86.
21.
Hoechel J, Lehmann D, Fehrenberg C, Unger V, Groneberg DA, Grosse-Siestrup C: Effects of different perfusates on functional parameters of isolated perfused dog kidneys. Nephrol Dial Transplant 2003;18:1748–1754.
22.
Grosse-Siestrup C, Nagel S, Unger V, Meissler M, Pfeffer J, Fischer A, Groneberg D: The isolated perfused liver: A new model using autologous blood and porcine slaughterhouse organs. J Pharmacol Toxicol Methods 2002;46:163.
23.
Grosse-Siestrup C, Fehrenberg C, Groneberg DA: Isolated hemoperfused porcine skin as a valid model to assess percutaneous absorption. J Invest Dermatol 2002;119:197–199.
24.
Groneberg DA, Grosse-Siestrup C, Fischer A: In vitro models to study hepatotoxicity. Toxicol Pathol 2002;30:394–399.
25.
Korb SM, Albornoz G, Light JA: Selenium addition to the flush/preservation solution protects kidneys against oxidative stress during warm and cold ischemia. Transplant Proc 1990;22:452–454.
26.
Herrero I, Torras J, Carrera M, Castells A, Pasto L, Gil-Vernet S, Alsina J, Grinyo JM: Evaluation of a preservation solution containing fructose-1,6-diphosphate and mannitol using the isolated perfused rat kidney. Comparison with Euro-Collins and University of Wisconsin solutions. Nephrol Dial Transplant 1995;10:519–526.
27.
Hauet T, Tallineau C, Goujon JM, Carretier M, Eugene M, Tillement JP: Efficiency of trimetazidine in renal dysfunction secondary to cold ischemia-reperfusion injury: A proposed addition to University of Wisconsin solution. Cryobiology 1998;37:231–244.
28.
Hauet T, Mothes D, Goujon J, Germonville T, Caritez JC, Carretier M, Eugene M, Tillement J: Trimetazidine reverses deleterious effects of ischemia-reperfusion in the isolated perfused pig kidney model. Nephron 1998;80:296–304.
29.
Goujon JM, Hauet T, Menet E, Levillain P, Babin P, Carretier M: Histological evaluation of proximal tubule cell injury in isolated perfused pig kidneys exposed to cold ischemia. J Surg Res 1999;82:228–233.
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