We investigated the expression and the N-formyl-methionyl-phenylalanine (fMLP)- and interleukin-8 (IL-8)-induced mobilisation of the complement regulatory proteins CR1 (CD 35), decay-accelerating factor (CD55) and CD59 on neutrophils in neonates and adults. The expression of CD35 on resting neonatal neutrophils was significantly higher than in adults. Both fMLP and IL-8 increased CD35 expression more markedly in adults. CD55 on resting neutrophils in neonates was significantly higher than in adults, but did not further increase upon fMLP stimulation as opposed to adults. The increase in CD59 expression was more pronounced in adult neutrophils and was significantly higher than in neonates after fMLP activation. The addition of the protease inhibitor phenylmethanesulphonyl fluoride during fMLP activation improved the upregulation of CD 3 5 significantly more in neonates, but not CD 55 or CD59. This effect could be replaced by human normal sera. These data demonstrate that differences exist between neonatal and adult neutrophils with respect to the expression and mobilisation of functional receptors related to protection from autologous complement lysis and may indicate differences in the efficiency to circumvent complement-mediated cell damage at the inflammatory site.

This content is only available via PDF.
Copyright / Drug Dosage / Disclaimer
Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.
You do not currently have access to this content.