The ability of granulocytes to generate superoxide anions (O2) and hypochlorite (OCL––) during phagocytosis was investigated using peripheral blood samples from adults and cord blood samples from neonates, using the chemiluminescence probe cypiridina luciferin analog (CLA) for O2 generation and luminol (L) for OCl–– generation. OCl–– generation by granulocytges was also monitored by taurine chloramine formation. The chemiluminescence probe based upon CLA was highly specific for and sensitive to O2 and could be adopted to determine O2 generation in terms of xanthine oxidase units. The CLA-dependent chemiluminescence by cord blood granulocytes was significantly higher than that by normal adult granulocytes. Taurine chloramine formation was significantly correlated with the Independent chemiluminescence (L-CL). Thus, the L-CL is considered to be mainly involved in OCl–– generated by phagocytizing granulocytes. L-CLs by cord blood granulocytes and normal adult granulocytes were essentially the same during phagocytosis.

Keywords:
Granulocytes, Superoxide anions, Hypochlorite, Cypiridina luciferin analog, Luminol, Chemiluminescence
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© 1990 S. Karger AG, Basel
1990
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