... an important role in mediating leptin signaling in the hypothalamus. We have demonstrated that leptin action in the hypothalamus is mediated by an insulin-like signaling pathway involving stimulation of PI3K (phosphatidylinositol-3 kinase) and PDE3B (phosphodiesterase-3B), and reduction in cAMP levels...

... and dibutyryl-cAMP induce PDE10A mRNA, the in vitro clock-dependent control of Pde10a appears to be mediated via a norepinephrine → β-adrenoceptor → cAMP/protein kinase A signaling pathway. With regard to the physiological role of PDE10A in the pineal gland, the specific PDE10A inhibitor papaverine was seen...

..., deiodinase type II (DII) activity, and cAMP content. Methods: We injected specific α- and β-adrenergic receptor (AR) agonists and antagonists along with an anti-thyroid drug to find out any AR-mediated action on central homeostasis . Results: The α 2 -AR agonist did not alter the onset of central homeostasis...

... via inhibition of calcium influx through voltage-sensitive channels. The cyclic AMP (cAMP) derivative 8-bromo-cAMP potentiated GnRH stimulation of LH release but did not prevent the melatonin-induced inhibition of LH release. However, when used in combination with Bay K, which reduced only partially...

..., instructions or products referred to in the content or advertisements. Gonadotropin-releasing hormone GT 1 cells cAMP cGMP Calcium Protein kinase C Regulation of Gonadotropins Ncuroendocrinology 1995:61:310-317 Gonzalo Martinez de la Escalera*h Amy L.H. Choia Richard I. Weiner* a Reproductive...

... receptor type I isoforms that transduce through the cAMP and inositol phosphate pathways. In neurointermediate lobe explants, PACAP 27 and PACAP 38 (10 –8 M ) stimulate cAMP accumulation whereas PACAP 38 but not PACAP 27 stimulates inositol phosphate breakdown. However, both ligands are potent stimulators...

...Anderson On-Lam Wong; Glen van der Kraak; John Philip Chang Previously, we have demonstrated that dopamine (DA) stimulates growth hormone (GH) release from the goldfish pituitary through DA D1 receptors. In the present study, the role of cAMP in DA D1-stimulated GH release was investigated using...

... with PACAP 27/38 led to a dose-dependent increase in cAMP content and ACTH accumulation in the medium with an apparent ED 50 value close to 10 –9 M The genomic effects of PACAP were first investigated by using a reporter gene containing a cAMP responsive element (CRE: TGACGTCA). PACAP 27/38 stimulate...

...), which are produced in the magnocellular nucleus of the hypothalamus. To study the role of sauvagine, cAMP, TRH and phorbol 12-myristate 13-acetate (PMA) in the regulation of POMC biosynthesis, the degree of incorporation of radioactive amino acids into the POMC protein was determined after treatment...

... determined the binding affinity of two series of analogues in which the entire sequence of hGRF(1-29)NH2 was scanned with D-amino acid and alanine substitutions. To further document their potency, we have evaluated the ability of representative candidates of each series to stimulate cAMP production...

...Tràn Vân Chuoï; Pierre Vacher; Bernard Dufy We have studied the effects of GnRH-associated peptide (GAP) on cAMP, cGMP production and intracellular calcium (Ca 2+ ) levels in the clonal rat GH 3 pituitary cell line. We show that GAP decreases cAMP production in a dose-dependent manner. A 20-min...

...Steven M. Gabriel; Carol M. Milbury; Steven M. Alexander; James A. Nathanson; Joseph B. Martin Growth hormone (GH) release and cAMP content were measured in monolayer cultures of anterior pituitary cells after β-adrenergic and GH-releasing factor (GRF) receptor activation. Isoproterenol (Iso, ED50...

... completely lost, suggesting that the dopaminergic inhibition is mediated by guanosine triphosphate binding proteins. 24 02 1987 15 07 1987 2 4 2008 Proopiomelanocortin Messenger RNA Dopamine Corticotropin-releasing factor cAMP Ca + + Pituitary Melanotrope cell...

...Gennaro Schettini; Eric L. Hewlett; Michael J. Cronin; Koji Koike; Takeshi Yasumoto; Robert M. MacLeod The dopaminergic inhibition of anterior pituitary adenylate cyclase activity, cAMP accumulation, and prolactin release was studied in the presence of the Ca 2+ channel activator, maitotoxin...

... receptor. In the present study, the role of cAMP in this response has been examined. Suspended pituitary cells from adult ovariectomized rats primed with estradiol were incubated with varying concentrations of LHRH or a highly active LHRH analog (LHRH-A) for 30 min at 37 °C, and levels of cAMP were...

... that it has no effect on 5-hydroxytryptamine metabolism. Melatonin increased CSF indoleacetic acid significantly, indicating increased metabolism of tryptamine. Melatonin did not affect CSF cAMP levels, but increased cGMP levels. The effect on indoleacetic acid and cGMP was seen in both lumbar and cisternal...

... and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements. ACTH Neurointermediate lobe Ions cAMP Neuroendocrinology JO: 262-267 (1980) In vitro Regulation of ACTH Release...

...B.A. Weissman; D.F. Johnson Pimozide and haloperidol, the dopaminergic antagonists, have been shown to effectively block diethylstilbestrol (DES)-elicited [ 3 H] adenosine 3’, 5’-monophosphate (cAMP) accumulation in incubated hypothalami from immature male rats. Several noradrenergic antagonists...

...H.J. Ruoff; R. Mathison; K. Lederis The presence and production of cyclic 3’, 5’-adenosine monophosphate (cAMP) were investigated in the hypothalamus and neural lobe of the rat. Theophylline (concentrations from 10 –3 to 8 × 10 –3 M) increased the in vitro content of cAMP in the isolated neural...

...R. Siegel; N. Conforti; S. Feldman; I. Chowers The effects of neurogenic and systemic stresses onhypothalamic and adenohypophysial cAMP content were studied. Acute neurogenic stress caused a rise in cAMP content in both organs. However, it was not significant when compared to the control animals...