... voltage-clamp and single-channel patch-clamp recording to determine directly the effects of EGF (10 n M for 48 h) on L-type calcium current density, the current-voltage relationship, single-channel amplitude, and opening and closing dwell times in rat GH4C1 pituitary tumor cells. Sustained, nimodipine...

... are still lacking. In the present report we studied the GABA-induced current responses in cultured cells from the anterior pituitary gland of 6- to 10-day-old rats using the patch-clamp technique in the whole cell configuration. Fast application of GABA (100 µ M ) induced membrane currents in 90% of cells...

...Philip M. Heyward; Chen Chen; Iain J. Clarke We have used conventional whole-cell patch-clamp to investigate the membrane currents of ovine anterior pituitary gonadotropes, and nystatin-perforated whole-cell patch-clamp to record the membrane potential changes elicited by the natural hypothalamic...

... oscillations were recorded indirectly by monitoring the activity of apamin-sensitive Ca 2+ -activated K + channels using the perforated patch-clamp technique and fast microperfusion system. Melatonin (1 n M ) inhibited the initiation or attenuated the amplitude of oscillatory current responses induced by 10 n...

... transient outward K + current appears to be important in the determining the resting potential of ovine somatotrophs and plays a major role in regulating basal intracellular Ca 2+ concentration and growth hormone secretion. K + currents Patch clamp Somatotrophs Ca 2+ Growth hormone 14 05...

...Jérôme Trouslard; Barbara A. Demeneix; Paul Feltz The electrophysiological properties of porcine melanotrophs in primary culture were studied with patch clamp techniques. In the cell-attached (c/a) configuration, extracellular spikes were recorded but in only 47 of the 259 cells examined; active...

...William T. Mason; Dennis W. Waring Gonadotrophs of the ovine pars tuberalis have been studied using the patch clamp technique for recording of single ion channel currents. We report that gonadotrophin-releasing hormone (GnRH) acts on these cells to open an inward-current cation channel which...

...Christopher J. Price; Willis K. Samson; Alastair V. Ferguson Neuropeptide W (NPW) is a ligand of the recently deorphaned receptor GPR7. Intracerebroventricular injection of this peptide results in reduced serum growth hormone concentration. Using whole-cell patch clamp recordings from somatostatin...

... in this species. Primary cultures of pituitary cells from maturing Atlantic cod were prepared by trypsin treatment and mechanical dispersion. Electrophysiological recordings were performed using the perforated patch clamp method. A subpopulation of large cells were selected for recordings. Spontaneous action...

... and metabolism, we studied the effect of orexin-B on voltage-gated Ca 2+ currents and the related signalling mechanisms in primary cultured ovine somatotropes using whole-cell patch-clamp techniques. With a bath solution containing TEA-Cl (40 m M ) and Tetrodotoxin (TTX) (1 µ M ), three subtypes of Ca 2...

... 10 2001 Gonadotropes Melatonin Melatonin receptors Gonadotropin-releasing hormone Calcium Development Patch clamp To elucidate the discrepancy between the actions of melatonin in neonatal gonadotropes and transfected cells, we examined the effect of melatonin on GnRH-induced [Ca...

... the specific role played by chloride in stimulus-secretion coupling. Lactotropes Cl – substitution Ca 2+ channels Prolactin Patch clamp In substitution experiments, NaCl was replaced by an equimolar (137 m M ) amount of one of the following Na + salts: methane sulfonate (CH 3 SO – 3 ), bromide...

... imaging and patch-clamp techniques. In order to test whether patch recording induces artificial alteration of the [Ca 2+ ] i oscillatory pattern, we recorded separately or simultaneously [Ca 2+ ] i and membrane potential. In the absence of any other stimulation, seal formation in patch-clamp recording...