..., especially in nonhuman primates. Therefore, we used a novel transmission scanning electron microscopy technique to examine the cytoarchitecture of the ME of young and aged female rhesus macaques in a preclinical monkey model of menopausal hormone treatments. Rhesus macaques were ovariectomized and treated...

... in the density of GPER-immunoreactive cells in the arcuate and periventricular nuclei of female rhesus monkeys and confirmed that GPER+ cells are found in regions where GnRH neurons are expressed in the monkey [ 28 ]. Considering that GPER can mediate actions of E 2 on GnRH cells, together with known aging...

.... How these two peptides interact to affect GnRH pulse generation remains a mystery. To address the hierarchy of the NKB and kisspeptin signaling pathways that are essential for GnRH release, two experiments were conducted using agonadal, juvenile male monkeys. Pituitary responsiveness to GnRH was first...

... and the mechanisms responsible for their synchronization are unknown. The purpose of the present study was to examine the role, if any, of GnRH in the functioning of the GnRH pulse generator in rhesus monkeys. Ovariectomized animals bearing recording electrodes chronically implanted in the mediobasal hypothalamus...

...Paul C. Goldsmith; James E. Boggan; Khushdev K. Thind Expression of hypothalamic estrogen receptors (ER) and progesterone receptors (PR) is barely evident in prepubertal monkeys but is prominent in adults. To investigate whether adult patterns of ER and PR expression are established in mid-pubertal...

... are known to affect peripubertal GnRH neurohormone release, but their relative roles remain unclear. In a developmental survey, estrogen receptors (ER) and progesterone receptors (PR) were virtually undetectable after immunostaining the hypothalamus of prepubertal monkeys, but were clearly evident...

...Melanie Pecins-Thompson; Andrea A. Widmann; Cynthia L. Bethea Progesterone (P) stimulates prolactin secretion through a neural mechanism in estrogen (E)-primed female monkeys. Several peptides, including β-endorphin (BE), oxytocin (OT), substance P (SP) and vasoactive intestinal polypeptide (VIP...

...Melanie Pecins-Thompson; Cynthia L. Bethea Progesterone (P) stimulates prolactin secretion through an unknown neural mechanism in estrogen (E)-primed female monkeys. Serotonin also stimulates prolactin secretion and this laboratory demonstrated that E induces nuclear progestin receptors (PR...

...Cynthia L. Bethea To test the hypothesis that estrogenic compounds may decrease the sensitivity of primate lactotropes to adenylate cyclase-mediated secretagogues, the effect of VIP on prolactin secretion and cAMP levels in serum-free monkey pituitary mono-layer cultures was examined...

...Pamela M. Gilbeau; Yoshio Hosobuchi; Nancy M. Lee This study examines the role of dynorphin-A(1-13) and dynorphin-A(1-10)-amide in the neuroendocrine regulation of anterior pituitary hormones in nonrestrained, adult male rhesus monkeys. The effects of these opioids on plasma concentrations...

...David R. Garris; Reinhart B. Billiar; Yoshiro Takaoka; Robert J. White; Brian Little A neurosurgical procedure has been developed for vascular isolation of the hypothalamus-thalamus region of the rhesus monkey brain. Utilizing this preparation, the left and right halves of the hypothalamus were...

...Reinhart B. Billiar; Yoshiro Takaoka; Wayne Johnson; Robert J. White; Brian Little A neurosurgical procedure has been developed for the vascular isolation of the hypothalamus-thalamus region of the rhesus monkey brain. The circulation to the left and right halves of the hypothalamus was also...

...L.S. Frawley; J.D. Neill Vasoactive intestinal polypeptide (VIP) is a potent stimulus for prolactin (PRL) release in rats. The purpose of this study was to test the effect of VIP on PRL secretion in rhesus monkeys and to identify its site of action. Three experimental models were used: (1) intact...

...David R. Garris; Rinhart B. Billiar; Yoshiro Takaoka; Robert White; Brian Little The in situ hypothalamic distribution of ( 3 Η)progesterone was studied in the vascularly separated and isolated hypothalamus of estrogen-treated, ovariectomized rhesus monkeys. The distribution of ( 3 H)progesterone...

...J.N. Hayward; K. Murgas; K. Pavasuthipaisit; F.R. Perez-Lopez; M.V. Sofroniew To evaluate a possible role of the amygdala (Amyg) in the neural control of arginine vasopressin (AVP) release, adult female monkeys ( Macaca mulatto ) with electrodes chronically implanted in the Amyg, hypothalamus...

...J.N. Hayward; K. Pavasuthipaisit The objective of this study was to determine the effects of i.v. nicotine on plasma arginine vasopressin (AVP), plasma osmolality, and behavior in the conscious monkey. Adult, female, chronically prepared monkeys (Macaca mulatta) were studied with i.v. infusion of 5...

...A.L. Ehle; J.W. Mason; L.L. Pennington Electrical stimulation of the amygdaloid nuclei in conscious monkeys sufficient to produce after-discharge was found also to produce a prompt and usually large increase in plasma growth hormone (GH) levels if prestimulation values were low and stable. Plasma...

...M. Miller; J.W. Wilks 24-hour urine collections were made in normally cycling and pregnant adult female rhesus monkeys. Volume and osmolality were measured and the antidiuretic hormone (ADH) excretion was determined by a radioimmuno-assay of extracted urine. No differences were found between pre...

...G.P. Smith; A.W. Root Plasma concentrations of immunoreactive growth hormone (GH) and of 17-hydroxycorticosteroids (17OH-CS) were measured during electrical stimulation of hypothalamus or hippocampus in 6 conscious monkeys adapted to chronic restraint in primate chairs. Plasma GH and 17OH-CS...

... monkeys with normal menstrual cycles. Plasma concentrations of SP were determined in blood samples taken every morning in normally menstruating cynomolgus monkeys throughout the menstrual cycle. There was a significant decreasing linear trend of SP during the follicular phase (cycle day –13 to day 0...