... in the mediobasal hypothalamus and the fenestrated capillaries of the hypothalamo-hypophysial portal system in the median eminence [ 5-7 ]. While still controversial, tanycytes would also contact other neural cells – including neurons – located in the arcuate nucleus, the ventromedial nucleus, and the dorsomedial...

... to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements. Caspase-3 Ki67 Subgranular zone Granule cell layer Median eminence Pars tuberalis Melatonin receptor subtype Cell proliferation, apoptosis, and the balance...

...Michelle M. Naugle; Sateria A. Lozano; Fay A. Guarraci; Larry F. Lindsey; Ji E. Kim; John H. Morrison; William G.M. Janssen; Weiling Yin; Andrea C. Gore The median eminence (ME) of the hypothalamus comprises the hypothalamic nerve terminals, glia (especially tanycytes) and the portal capillary...

... of both melatonin receptor types (MT1 and MT2) were adapted to a 12-hour light, 12-hour dark photoperiod and were sacrificed at ZT00, ZT06, ZT12, and ZT18. Immunohistochemistry for Ki67 and activated caspase-3 served to identify and quantify proliferating and apoptotic cells in the median eminence (ME...

... regions. Human Hypothalamus Infundibulum Median eminence Pituitary Reproduction Tachykinins Peroxidase-labeled sections were analyzed and representative light microscopic images prepared with an AxioCam MRc 5 digital camera mounted on a Zeiss AxioImager M1 microscope, and using...

... 2 , E 2 + progesterone, or vehicle) selected to mimic currently prescribed hormone replacement therapies used for the alleviation of menopausal symptoms in women. We found that about half of GnRH perikarya co-expressed GPER, while only about 12% of GnRH processes and terminals in the median eminence...

... light on the control of GnRH neuron excitability and GnRH secretion. This work suggests that GnRH neurons have a unique projection to the median eminence that possesses both dendritic and axonal properties. We propose that this ‘dendron' projection allows GnRH neurons to multitask and integrate...

... of the median eminence (ME) after counterstaining of nuclei with Hoechst (white). Boxed areas indicate the site of the images shown in j and k . j , k Tanycytes, whose cell bodies line the ventricular wall (arrowheads), send processes toward the capillaries (Cap) of the pituitary portal blood system. GnRH...

... preoptic area of intact but not castrated goats. Kisspeptin-ir cell bodies and fibers in the arcuate nucleus (ARC) and median eminence (ME) were fewer in intact male goats compared with castrated animals. Apposition of kisspeptin-ir fibers on GnRH-ir cell bodies was very rare in both intact and castrated...

... immunocytochemical and mRNA analysis. Adult male rats were subjected to 72 h of food deprivation, during which an average of 18% total body weight was lost. ICC studies were performed on brain sections from the rostral, middle and caudal regions of the median eminence of the hypothalamus using the avidin-biotin...

... to investigate the effects of galanin on both growth (GH) and luteinizing hormones (LH) from pituitaries of young male calves. Pituitary slices (P, 500 µm in thickness) were perfused alone or coincubated with median eminence terminals (ME) in DMEM-F12 plus BSA 0.1% and antibiotics. The perifusion chambers were...

... nucleus and the latter from cell bodies in the zona incerta and lateral hypothalamus. Within the hypothalamus they innervate the pre-optic area, median eminence (ME) and ventromedial nucleus (VMN). Both peptides stimulate sexual behaviour and in this report we have investigated their effect on another...

...Maria Isabel Gonzalez; Pamela Greengrass; Michael Russell; Catherine A. Wilson In a previous study we have shown that a positive correlation exists between 5-hydroxytryptamine (5-HT) activity and female sexual receptivity in the pre-optic area (POA) and median eminence (ME...

... from hypothalamic tissue of male rats, and whether these effects could be modulated by testosterone (T). Mediobasal hypothalamic (MBH) or median eminence (ME) fragments from either sham-operated or castrated male rats (7 days) were placed in superfusion chambers and superfused with M199 for a 30-min...

...Corinne Estupina; Albin Pinter; Jorge Belmar; Hélène Astier; Sandor Arancibia To investigate the secretory pattern of somatostatin (SS) from the median eminence (ME) in the female rat, as well as estrogenic influence on this secretion, we measured both SS release and hypothalamic content in cycling...

... glands collected for the quantification of GH and LHβ mRNA by Northern analysis. Three HPD wethers and 1 HPD ewe and 1 control ewe were killed and their brains were perfused; the hypothalami were sectioned and immunostained for the presence of GRF-containing fibres in the median eminence. Normal episodic...

... monitoring plasma ACTH levels. Perfused hypothalamic areas were the median eminence (ME), the paraventricular nucleus (PVN), and the medial preoptic area (MPOA). During the period 12:00-15:00 h, perfusates and blood samples were collected every 10 min (between 13:00 and 13:40 h) or 20 min (between 12:00...

...Hajime Watanobe; Kazuo Takebe In order to assess the physiological role of neurotensin (NT) in regulating the secretions of luteinizing hormone (LH) and prolactin (PRL), we examined whether the in vivo release of NT from the push-pull cannulated median eminence (ME) was altered in association...

... isolated median eminence (ME) alone. After 1 h stabilization periods, eight MEs released GnRH in an episodic pattern during 4-hour experiments (samples collected every 4 min, RIA in triplicate) with mean (± SE) release rates of 2.2 ± 0.2 pg GnRH/4 min and peak amplitudes of 1.3 ± 0.2 pg/4 min...

... of neurosecretory vesicles from immunocytochemically identified axons in the external zone of the median eminence. The habituated, vehicle-injected sham control group had normal levels of plasma ACTH and corticosterone, but possessed a significantly higher proportion of VP-containing CRH axons than naive animals...