.... To compare where recombination takes place, we crossed the Gnrh-cre and Lhrh-cre lines with the Rosa26-LacZ reporter mouse. Lhrh-cre allowed recombination of the Rosa26-LacZ gene in ∼700 cells, which is comparable to the GnRH neuronal population. Surprisingly, there were > 20 times more LacZ expressing...

.... Gonadotropin-releasing hormone Hypothalamus Immunohistochemistry Kisspeptin LHRH Neurokinin B Reproduction Hypothalamic kisspeptin (KP)/KP receptor signaling is critical for mammalian puberty and fertility [ 1, 2 ]. The topography, morphology, connectivity, and plasticity of human KP neurons...

... or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements. GABA GABA turnover Gonadal steroids LHRH Hypothalamus, rat...

...Ann M. Contijoch; Sasha Malamed; Dipak K. Sarkar; Juan-Pablo Advis We studied the effect that β-endorphin (βEND) might have at the median eminence (ME) on luteinizing hormone (LH)-releasing hormone (LHRH) during the ovulatory cycle of domestic hens. Thus, we assessed (a) the immunocytochemical...

...Gabriela González-Mariscal; Angel I. Melo; Carlos Beyer In experiment I we studied the capacity of progesterone (P) and two nonsteroidal agents that activate lordosis, but do not bind to the progestin receptor (PR), i.e. luteinizing hormone-releasing hormone (LHRH) and prostaglandin E 2 (PGE 2...

... removable probe in additional discrete hypothalamic and extrahypothalamic areas. To determine the capabilities of this sampling technique, we assessed in vivo release of LHRH from the extracellular space at the posterior-lateral ME, under various reproductive conditions that have well-defined LH-secretory...

...Andrea C. Gore; Dai Mitsushima; Ei Terasawa The onset of puberty is heralded by an increase in pulsatile LHRH release. Since neuropeptide Y (NPY) has been implicated as a major regulator in the control of pulsatile LHRH release in mature monkeys, we have hypothesized that maturational changes...

...Diane F. Matesic; John A. Germak; Emmanuel Dupont; Burra V. Madhukar Expression of gap junction proteins was studied in the LHRH neuronal cell line, GT1-7, as a first step in defining the signalling mechanisms responsible for the pulsatile secretion of LHRH. GT1-7 cells were found to express...

...James L. O’Conner; Marlene F. Wade; Darrell W. Brann; Virendra B. Mahesh Neuropeptide Y (NPY) acts at the hypothalamus to stimulate LH secretion through increased LHRH secretion. NPY is also cosecreted into the portal system in a pulsatile manner with LHRH and may act at the anterior pituitary...

...Jean-Pierre Gautron; Eliane Pattou; Pierre Leblanc; L ‘Héritier; Claude Kordon Several molecular forms related to the decapeptide LHRH were characterized and quantified in various brain structures of intact and castrated male and female rats. Distinct moieties were separated by high performance...

...Toshihiko Inukai; Xiangbing Wang; Monte A. Greer; Susan E. Greer We utilized luteinizing hormone-releasing hormone (LHRH) secretion by perifused minced rat hypothalamic median eminence (ME) tissue to evaluate whether isotonic ethanol would stimulate neurosecretion as it does secretion from...

...Marianne K. Steele; Kate N. Stephenson; John M. Meredith; Jon E. Levine The present experiments assessed the effects of central administration of angiotensin II (Ang II) on mean levels of luteinizing hormone-releasing hormone (LHRH) in the extracellular fluid of the anterior pituitary gland...

... 4 2008 Aging Reproductive system LHRH Melatonin Pineal gland Opioid system Naloxone © 1992 S. Karger AG, Basel 1992 Copyright / Drug Dosage / Disclaimer Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized...

... and 8 µg). On the other hand, plasma prolactin was unchanged after intraventricular injection of o-FSH. Since the pituitary responsiveness to synthetic LH-releasing hormone (LHRH) was unimpaired at the end of the sampling and incubation with o-FSH suppressed the release of LHRH from median eminence...

...Duncan W.F. Porter; Dennis W. Lincoln; Alasdair M. Naylor The delayed and sustained suppression of LH secretion induced by the administration of LHRH into the third cerebral ventricle of the ovariectomized ewe suggests the activation of a neuroendocrine mechanism involving components separate from...

...Robert L. Moss; Carol A. Dudley Both the luteinizing-hormone-releasing hormone (LHRH) decapeptide and an LHRH fragment consisting of the last 6 amino acids of the decapeptide, Ac-LHRH 5–10 , have been shown to enhance lordosis behavior in ovariectomized, estradiol-benzoate treated female rats...

...Alasdair M. Naylor; Duncan W.F. Porter; Dennis W. Lincoln The role of central luteinizing hormone releasing hormone (LHRH) in the control of pulsatile LHRH and luteinizing hormone (LH) secretion was investigated in ovariectomized adult ewes. Injection of LHRH (2.1–21 pmol) into the third cerebral...

... (LHRH) between rats infused with saline or naloxone at any stage of pregnancy, demonstrating that the stimulatory effect of naloxone was not exerted at the pituitary level. Naloxone increased both the amplitude and frequency of pulsatile LH secretion on days 6–8, and stimulated frequency on days 14–16...

... injected bilaterally into the MCG in ovariectomized, estrogen-treated females, and the lordosis response was recorded at repeated intervals. In the first experiment, three doses of SP (50, 500 and 1,000 ng/cannula), a single dose of LHRH (50 ng/cannula) or vehicle were given to separate groups of females...

...)-releasing hormone (LHRH). LH release showed a characteristic pattern of prolonged (over 5 h) sensitisation to the releasing hormone, with the response to each volley becoming progressively greater. The addition of 2 m M difluoromethylornithine (DFMO; an inhibitor of ornithine decarboxylase...