... or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements. Hindbrain RedIL6 Immunohistochemistry In situ hybridization...

... products have not been identified yet. Therefore we studied the expression of several adenohypophyseal hormones and their subunits (TSH, FSH, LH, common α-chain, GH, ACTH, PRL, α- and γ-MSH, β-lipotropin) by immunocytochemistry, Northern blot analysis and in situ hybridization in the sheep pituitary. Only...

...Sandra Ceccatelli; Lindsey Grandison; Roderick E.M. Scott; Donald W. Pfaff; Lee-Ming Kow Expression and estrogen regulation of the genes for nitric-oxide (NO)-synthesizing enzymes (NO synthase, NOS) were investigated by in situ hybridization. This study focused on regions of the hypothalamus...

... is ubiquitously expressed and the protein is under translational control. To determine the ontogeny and localization of Pit-1 expression in the human fetus, we examined fetal pituitaries (n = 23) at various stages of gestation from 6 weeks to term using in situ hybridization (ISH) for pit-1 mRNA...

... immunoreactivity, both in vasopressin and oxytocin neurons. Using in situ hybridization and immunohistochemistry we have also investigated the expression of the mRNAs for oxytocin, dynorphin, galanin and galanin message-associated peptide and of oxytocin and dynorphin in the PVN of lactating and nonlactating rats...

...) neurons some of which coexpress dopamine, detected by immunostaining for tyrosine hydroxylase (TH). The present immunocytochemical, in situ hybridization and retrograde-labeling studies show that each TIDA neuron of pregnant, pseudopregnant, lactating, and aged female rats contains ENK-like...

... throughout the brain of CORT-treated compared with ADX rats by in situ hybridization; the pattern of expression is similar to that of proteolipid protein mRNA and is consistent with a predominant expression in oligodendrocytes in white matter. Restraint and cold stress also increased GPDH mRNA...

... and in situ hybridization histochemistry to study CCK B receptor mRNA in hypothalamic neurons. Changes in the expression of CCK B receptor mRNA in the SON and PVN were analysed in salt-loaded as well as in hypophysectomized animals. Levels of CCK B receptor mRNA in the PVN and SON increased markedly in salt...

...) of the hypothalamus and the effect of AII on corticotropin-releasing hormone (CRH) and vasopressin (VP) mRNA levels. In situ hybridization studies using cRNA 35 S-labelled probes showed that while type 1 AII receptor (AT 1 ) mRNA levels were high in the periventricular and parvicellular pars of the PVN, only very low...

... was determined by in situ hybridization using a full-length riboprobe followed by quantification with a computer-assisted image analysis system. Cultures derived from female rats contained significantly (p < 0.01) higher amounts of TRH and secreted approximately twice (p < 0.01) as much TRH under basal...

... have investigated the effects of GAL on the vasopressin (AVP) mRNA content in the hypothalamic magnocellular neurons. We demonstrated by in situ hybridization (ISH) and immunohistochemistry that 100 pmol of GAL or GAL fragment (1-16) injected into the lateral ventricle of anesthetized dehydrated male...

... tails were found in MCH RNAs isolated from the peripheral organs by comparison with hypothalamus MCH RNAs of Wistar rats. In order to localize MCH expression in gastrointestinal and genital tracts of Wistar rats we performed in situ hybridization with specific 33 P-labeled oligo-probes joined...

... receptor In situ hybridization Stress Corticotropin and Proopiomelanocortin Regulation Neuroendocrinology 1995;61:180-190 James P. Herman David Adams Chantal Prewitt Department of Anatomy and Neurobiology, University of Kentucky Medical Center, Lexington, Ky., USA Regulatory Changes in Neuroendocrine...

...-field stressor. In situ hybridization histo-chemistry with 35 S-labeled oligonucleotide probes was followed by quantitative cell by cell autoradiography. When basal CRF and VP mRNA levels were compared in the two lines, we found that the RLA rats had a significantly higher VP-labeling density than...

...: neither hypothalamic nor pituitary levels of GAL mRNA were altered by insulin treatment. Adrenal galanin mRNA levels were maximally increased by 4 h, remained maximally elevated for at least 48 h, and had returned to baseline 6 days after insulin administration. In situ hybridization analysis localized...

...Matthijs A. Zandbergen; Olivier Kah; Jan Bogerd; Jan Peute; Henk J.Th. Goos The expression of prepro-catfish GnRH mRNA and prepro-chicken GnRH-II mRNA was investigated by means of in situ hybridization. The differential distribution of cells expressing the respective mRNAs was compared...

..., and at selected days of postnatal development, to determine the effects of GH deficiency on SRIH neuron development. DF/? and df/df mice of both sexes at postnatal ages 1, 3, 7, 14, 21 and 60 days (adult) were examined. In situ hybridization and image analysis were used to quantify the relative abundance of total...

..., restoration of the suckling stimulus by return of the pups provoked an increase in POMC gene expression which seemed to depend on high endogenous levels of PRL. 19 09 1995 7 02 1996 9 4 2008 Suckling Gene expression Lactation Arcuate nucleus In situ hybridization Neuropeptide Y...

... and were killed 3 h later (12.00, 15.00, 18.00 h). Frozen brains were mounted on a microtome, cut in 30-µm slices and then processed for the detection of c- fos mRNA and CRH primary transcript (heteronuclear [hnRNA]) by means of in situ hybridization histochemistry using 35 S-labeled exonic and intronic...

...), as well as the caudate putamen (CPu), CA1 region of the hippocampus, anterior pituitary, and uterine tissue of ovariectomized (OVX) female rats. By in situ hybridization we observed a 4.4-fold increase in oxytocin receptor mRNA levels in the VMH after 48 h of estrogen treatment when compared to OVX rats...