...John K. McDonald; James I. Koenig; Daniel M. Gibbs; Paulette Collins; Bryan D. Noe Pituitary portal blood was collected from urethane-anesthetized rats and examined for the presence of neuropeptide Y (NPY) using high-performance liquid chromatography and radioimmunoassay. Other rats were perfused...

..., except in the case of anterior pituitary content of ir-dynorphin 1–17 which fell progressively between 3 and 18 months (from 1.65 ± 0.15 ng/tissue at 3 months to 0.73 ± 0.12). Radioimmunoassay following high-performance liquid chromatography analysis of extracts of the tissues showed little variation...

...-melanotropin. The DSIP immunoreactive cells in the adrenal medulla were identical with a major population of the noradrenaline-storing cells. High-performance liquid chromatography of tissue extracts revealed one major peak of DSIP-like immunoreactivity which did not coelute with synthetic DSIP...

... characterized by reverse-phase high-performance liquid chromatography (RP-HPLC). In each case the major peak of immunoreactivity co-migrated with synthetic AVP. By peroxidase-antiperoxidase immunocytochemistry, sparsely distributed cells containing ir-AVP were localized in anterior pituitary sections. Levels...

... and Female Rats throughout Postnatal Development Alan G. Watts, Helen F. Stanley MRC Brain Metabolism Unit, University Department of Pharmacology, Edinburgh, Scotland Keywords. Hypothalamus Raphe area High-performance liquid chromatography Indoleamines NSD 1015 5-HT turnover Sexual...

...-hydroxytryptamine (5-HT) and also of the specific metabolites of NE, DA, and 5-HT, 3-methoxy-4-hydroxyphenylethylene glycol, 3,4-dihydroxyphenylacetic acid, and 5-hydroxyindole-3-acetic acid, respectively, were concurrently measured in microdissected nuclei using high-performance liquid chromatography...

...Kozo Hashimoto; Kazuharu Murakami; Norihito Ohno; Jingo Kageyama; Teruhiko Hattori; Yoshiyuki Aoki; Jiro Takahara Reversed-phase, high-performance liquid chromatography (HPLC) was carried out to characterize rat hypothalamic corticotropin-releasing factor (CRF) using synthetic ovine CRF as a marker...