...-releasing hormone (GnRH) release from the hypothalamus increases for a prolonged period of time, driving the adenohypophysis to increase the pulsatile release of luteinizing hormone with diurnal periodicity. Increased pubertal GnRH secretion does not appear to be driven by inherent changes in GnRH neuronal...

... regulating AMHR2 expression in these extragonadal sites remain to be explored. Method/Results: Here, we demonstrated in perifused murine LβT2 gonadotrope cells that Amhr2 expression is differentially regulated by GnRH pulse frequency with an induction under high GnRH pulsatility. Furthermore, we showed...

... B1 KO) show altered Gnrh1 and Gad1 expressions in the preoptic area-anterior hypothalamus (POA-AH) and females display disruption of cyclicity and fertility. Here we addressed whether sexual differentiation of the brain and the proper wiring of the GnRH and kisspeptin systems were already disturbed...

... determinations was confirmed by assessment of rat serum samples of known hormone concentrations, used as external controls. GnRH concentrations in the incubation medium were measured in 100-µl aliquots using a commercial RIA kit from Peninsula Laboratories (Bachem Group, San Carlos, Calif., USA), following...

... in that it is characterized by enhanced FSH secretion and FSH responsiveness to exogenous GnRH (vs. LH) from the gonadotrope, yet the mechanism of these dynamics remains unclear. This study aimed to elucidate this phenomenon using a human disease model of GnRH deficiency (idiopathic hypogonadotropic hypogonadism, IHH...

... hormone (GnRH). A link between nutrition and reproductive function is well established. In this study we investigated the effects of two peptides associated with appetite control, galanin and leptin, on the regulation of LH release from the pituitary. Methods: Using perifused anterior pituitary tissue...

...Gabriela T. Pérez; Marta E. Apfelbaum In the present work, the effects of GnRH on the translation (by [ 14 C]leucine incorporation; [ 14 C]Leu-LH) and the glycosylation of LH by rat pituitary cells in primary culture were established. The use of specific markers as radioactive precursors made...

...Ann-Judith Silverman; Joan W. Witkin In an attempt to understand the regulatory events that control the synthesis of gonadotropin-releasing hormone (GnRH) during the estrous cycle of the rat, we undertook a cellular analysis of time of translation of GnRH mRNA into protein. A specific antiserum, Rb...

... was to clarify the neuroendocrine and the gonadal changes following chronic intermittent stress in male rats and the action of a neuroactive drug, acetyl- l -carnitine (ALC). The effect of two different stressors, cold water swimming or ether, on central β-endor-phin (β-EP) and GnRH contents, and on plasma...

...Kelli A. Sullivan; Ann-Judith Silverman The origin of gonadotropin-releasing hormone (GnRH) neurons in the olfactory placode and their subsequent migration into the central nervous system (CNS) has been described in several species. The current study was undertaken to delineate the time course...

...Gonzalo Martínez de la Escalera; Amy L.H. Choi; Richard I. Weiner The mouse GT 1 gonadotropin-releasing hormone (GnRH) neuronal cell lines exhibit highly differentiated properties of GnRH neurons. This report investigates the direct effect of γ-aminobutyric acid (GABA) and subtype selective GABA...

...Winfried G. Rossmanith; Sylvia Boscher; Uwe Ulrich; Reiner Benz While a nocturnal rise accounts for the marked circadian variability of prolactin (PRL) secretion in humans, the mechanisms subserving this neuroendocrine manifestation are still obscure. Since gonadotropin-releasing hormone (GnRH...

...Rudraiah Medhamurthy; Vernon L. Gay; Tony M. Plant The purpose of the present study was to examine whether repetitive intravenous injections of L -glutamic acid (Glu), like those of N-methyl- D, L -aspartic acid (NMA), are able to elicit a sustained train of gonadotropin releasing hormone (GnRH...

...Thomas S. King; Inn Soo Kang; Martin A. Javors; Robert S. Schenken The purpose of this study was (1) to characterize more fully the effects of phorbol esters to stimulate GnRH release in vitro and (2) to determine whether cocaine (which disrupts estrous cyclicity in rats) affected phorbol ester...

...Matti Bergendahl; Jeffrey N. Wiemann; Donald K. Clifton; Ilpo Huhtaniemi; Robert A. Steiner Dietary restriction reduces circulating gonadotropin and testosterone levels in male rats, an effect thought to be mediated through reduced gonadotropin-releasing hormone (GnRH) secretion; however...

... cyclicity and ovulation rates and (2) the stimulated in vitro release of hypothalamic GnRH and aminergic neurotransmitters directly involved in regulating or modulating GnRH release. Within 7 days of treatment with 10 mg kg –1 day –1 of cocaine HC1 subcutaneously, rats demonstrated significant estrous cycle...

...Jean-D. Lalau; Michel L. Aubert; Danielle F. Carmignac; Isabelle Grégoire; Jean-Paul Dupouy A gonadotropin-releasing hormone (GnRH) antagonist, when injected 24 h before sacrifice to rat fetuses, did not modify plasma testosterone concentrations in males on day 18 of gestation but it did on days 19...

... at birth induced 1 h later a slight but not significant increase in hypothalamic gonadotropin-releasing hormone (GnRH) and pituitary luteinizing hormone (LH) contents, reduced drastically plasma LH levels and completely prevented the postnatal surge of plasma testosterone which occurred normally...

...Gordana Leposavic; Michael R. Dashwood; Jean Ginsburg; Julia C. Buckingham Adrenergic mechanisms have been widely implicated in the regulation of GnRH secretion in adult rats but their role in young animals, in which the activity of the GnRH neurones is minimal, is unclear. These experiments were...

...Cynthia L. Williams; Masugi Nishihara; Jean-Christophe Thalabard; Peter M. Grosser; Julane Hotchkiss; Ernst Knobil The effect of corticotropin-releasing factor (CRF) on the hypothalamic gonadotropin-releasing hormone (GnRH) pulse generator, the central neuronal system governing pulsatile pituitary...