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1-9 of 9
Keywords: GT1–7 cells
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Journal Articles
Journal:
Neuroendocrinology
Neuroendocrinology (2012) 96 (2): 162–171.
Published Online: 08 June 2012
... of intracellular signaling pathways (i.e. nonclassical actions). The mPRs are highly expressed in the brain, but research on their neural functions has only been conducted in a single neuronal cell line, GT1–7 cells, which have negligible nuclear progesterone receptor (PR) expression. GT1–7 cells express mPRα...
Journal Articles
Journal:
Neuroendocrinology
Neuroendocrinology (2009) 90 (3): 251–259.
Published Online: 30 July 2009
...–7 cells), the present study investigated the mechanism by which the expression of melatonin receptors is regulated in GnRH neurons. Like endogenous GnRH neurons, GT1–7 cells express both GnRH and GnRH receptor mRNAs, indicating that the cells have a self-stimulatory system. A 2-iodomelatonin binding...
Journal Articles
Journal:
Neuroendocrinology
Neuroendocrinology (1995) 61 (5): 499–508.
Published Online: 09 April 2008
... responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements. Calcium γ-Aminobutyric acid Glutamate Gonadotropin-releasing hormone GT1-7 cells Organization and Regulation of Hypothalamic Neurons...
Journal Articles
Jessica S. Jacobi, Cecilia Martin, Gabriel Nava, Michael C. Jeziorski, Carmen Clapp, Gonzalo Martínez de la Escalera
Journal:
Neuroendocrinology
Neuroendocrinology (2007) 86 (4): 260–269.
Published Online: 29 August 2007
... indirect effects of estradiol on GnRH neurons have been clearly demonstrated, direct actions are still controversial. In the current study, we examined direct effects of 17β-estradiol upon the expression of receptors for afferent signals at the level of the GnRH neuron, using immortalized GT1-7 cells...
Journal Articles
Cecilia Martin, Jessica S. Jacobi, Gabriel Nava, Michael C. Jeziorski, Carmen Clapp, Gonzalo Martínez de la Escalera
Journal:
Neuroendocrinology
Neuroendocrinology (2007) 85 (4): 257–266.
Published Online: 04 June 2007
... here were designed to explore the potential signaling pathways involved in this novel GABA A ionotropic inhibition of cAMP synthesis in GT1–7 cells. Using RT-PCR and real-time PCR, we found that GT1–7 cells express 8 of 9 known membrane adenylyl cyclase (AC) isoforms, including a large proportion...
Journal Articles
Journal:
Neuroendocrinology
Neuroendocrinology (2007) 84 (6): 353–363.
Published Online: 30 March 2007
.... Orexin-1 receptor (OX1R) expression was detected in GT1-7 cells by RT-PCR and Western blot. Results showed that 0.1–1 n M orexin A, when administered in culture media for 4 h, can significantly stimulate GnRH mRNA expression in GT1-7 cells (p < 0.05). Administration of 1 µ M OX1R antagonist, SB-334867...
Journal Articles
Journal:
Neuroendocrinology
Neuroendocrinology (2004) 80 (1): 2–10.
Published Online: 15 October 2004
... ]. Infection of GT1-1 cells with PDE adenovirus vector (Ad) increased the PDE activity in the cells and prevented the FSK-induced secretion of GnRH [ 5 ]. FSK directly stimulates the adenylate cyclase activity independent of a receptor-mediated event. We show that infection of GT1-7 cells with PDE-Ad...
Journal Articles
Journal:
Neuroendocrinology
Neuroendocrinology (1999) 70 (4): 246–254.
Published Online: 14 October 1999
... receptor types I, IB, and II. We examined the effects of activin A on GnRH protein secretion and mRNA levels in GT1-7 cells. Treatment with rh-activin A regulated both GnRH protein secretion and GnRH mRNA expression in the GT1-7 cells in a time-dependent fashion. Using transient transfection assays, we...
Journal Articles
Journal:
Neuroendocrinology
Neuroendocrinology (1999) 69 (6): 397–407.
Published Online: 14 June 1999
... and GluR6 mRNA, while the pituitary showed the presence of NMDAR1, GluR1 and GluR6 mRNA. Western blot analysis also showed the presence of NMDAR1 and GluR1 protein. Since there are relatively few GnRH neurons in the hypothalamus, and GT1-7 cells have been considered to be a GnRH neuronal cell line, GT1-7...