... immunocytochemistry of vasopressin (AVP) and corticotropin-releasing factor (CRF) in the external zone of the median eminence showed that prior footshock exposure increased the AVP i stores to 167% as compared to C rats, whereas CRF i content was not changed. In addition, S rats showed increased mineralocorticoid (MR...

.... Immobilization Pituitary Renin CRF Rats Original Paper Neuroendocrinology 1993 ;57:517-524 Thackery S. Gray Rashel A. Piechowski" Joseph M. Yrachetah Peter A. Rittenhouseh Cynthia L. Bethea1 Louis D. Van de KaV Departments of ' Cell Biology, Neurobiology and Anatomy l' Pharmacology, Loyola Stritch...

...Cynthia L. Williams; Masugi Nishihara; Jean-Christophe Thalabard; Peter M. Grosser; Julane Hotchkiss; Ernst Knobil The effect of corticotropin-releasing factor (CRF) on the hypothalamic gonadotropin-releasing hormone (GnRH) pulse generator, the central neuronal system governing pulsatile pituitary...

...Lydie Jeandel; Alain Van Dorsselaer; Bernadette Lutz-Bucher; Bernard Koch The present study attempts to determine whether part of the corticotropin-releasing factor (CRF)-like materials present in the ‘posterior pituitary’ is composed of authentic CRF and examines whether the concentration...

...Taiichiro Okajima; Georg Hertting Bradykinin (10 –6 and 10 –5 M ) stimulated ACTH-IR release from rat anterior pituitary tissue in vitro concentration-dependently. The onset of this effect was delayed in comparison to that of AVP or CRF. The combined treatment of bradykinin with AVP or CRF produced...

...Marco Gambacciani; Samuel S.C. Yen; Dennis D. Rasmussen The effects of corticotropin-releasing factor (CRF) on GnRH release from the adult male rat mediobasal hypothalamus (MBH) and median eminence (ME) were studied in an in vitro incubation system. CRF induced a dose-related inhibition of GnRH...

... in response to ether inhalation. In vitro NOR did not affect the basal or the CRF-induced stimulation of ACTH release. The present data are consistent with an α-stimulatory effect of NOR on the neuroendocrine pathways involved in the pituitary response of the newborn rat to ether stress and suggested...

... or CRF-induced β-end release. The effect of serotonin and melatonin was further tested in a superfusion system of dispersed rat anterior pituitary cells. Superfusion with oCRF (200 nmol/l) for 4 min elicited an immediate rapid increase in β-end release which lasted 30–40 min. Simultaneous superfusion...

..., dexamethasone. The present studies help to define the site of inhibition of both corticosterone and dexamethasone on the hypothalamus-pituitary-adrenal axis. Infusion of 20 or 202 µg corticosterone over 6 h significantly reduced hypothalamic CRF (corticotropin-releasing factor) content, whereas it did...

...G. Pelletier; L. Désy; J. Côté; G. Lefèvre; H. Vaudry; F. Labrie Using the peroxidase-antiperoxidase technique, we performed the ultrastructural localization of corticotropin-releasing factor (CRF)-like immunoreactivity in the rat hypothalamus. It could be demonstrated that CRF-like...

...-releasing factor (CRF) extracts or dbcAMP, in a manner which was both dose- and time-dependent. A close correlation appeared to exist between the degree of ACTH blockade and the percentage of filled steroid-binding sites. However, exposure of hypophyses to CORT for a critical period of time...

...B. Briaud; A. Enjalbert; C. Mialhe; C. Kordon Subcellular fractionation of the mediobasal hypothalamus (MBH) and frontal cerebral cortex was performed by differential and discontinuous sucrose gradient centrifugation. Corticotropin-releasing factor (CRF) activity of the different fractions...

...J.-L. Bény; A.J. Baertschi In diabetes insipidus (DI) rats, electrical stimulation of the posterior pituitary lobes in vitro promotes the release of corticotropin-releasing factor (CRF). The CRF activity is abolished by preincubation of the posterior lobe media with thioglycolate. Oxytocin, which...

...J.-L. Bény; A.J. Baertschi Electrical stimulation of rat posterior lobes in vitro inhibited bioactive corticotropin (ACTH) release from the intermediate lobe and promoted the release of corticotropin-releasing factor(s) (CRF). Both effects were calcium dependent. Released posterior lobe CRF...

...Frances Pearlmutter; Linda A. Dokas; Bonnie Loeser; Ann Kong; Murray Saffran; William Simmons Most of the corticotrophin-releasing factor (CRF) activity of normal rat median eminence (ME) extract binds to a neurophysin affinity column. The bound material contains the large and small factors, which...

..., or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements. CRF...

...A. Frances Pearlmutter; Eloise Rapino; M. Saffran The CRF-like activity of peptides related to vasopressin and oxytocin was estimated using a semi-automated in vitro bioassay. Rat pituitary halves were incubated for 1 h with the test material in 1.0 ml of Krebs-Ringer bicarbonate buffer. The ACTH...

...B. Koch; B. Bucher; C. Mialhe The possible binding of 3 H-dexamethasone (Dx)to the anterior pituitary and the effect of the steroid on neo-synthesis and release of ACTH of glands incubated with crude CRF extracts were examined. Neosynthesis of ACTH was determined by immunoprecipitation after...

... lysine nor arginine vasopressin stimulated ACTH release from the PI; and (d) a partially purified CRF preparation, which will stimulate ACTH release from the PD, did not alter ACTH release from the PI. We conclude that the hypothalamus contains a substance(s) that will stimulate ACTH release from the PI...

... the encephalectomized fetuses were given an injection of hypothalamic extract. This extract was devoid of appreciable ACTH activity when tested in decapitated fetuses. Cortical extract was inactive in encephalectomized or decapitated fetuses. These data suggest that fetal hypothalamic extract contains some CRF activity...