... the hypothalamus. However, no attempt has been yet made to determine whether CA affects GnRH gene expression. To this end, the effect of 6-hydroxydopamine (6-OHDA), a catecholaminergic neurotoxin, on GnRH mRNA level was examined. Hypothalamic tissues obtained from adult male rats were incubated with medium...

... innervation, we wanted to determine the role of catecholamines in the neuroendocrine response to stress. The stressor was a conditioned emotional (fear) response paradigm (CER). The catecholamine neurotoxin, 6-hydroxydopamine (6-OHDA), was injected into the PVN 14 days before the rats were subjected...

... (ACTH) secretion, we have measured CRF levels in rat hypophysial portal blood (HPB) after the pharmacological destruction of the ventral noradrenergic bundle (VNAB), using 6-hydroxydopamine (6-OHDA) stereotaxically injected into the VNAB. CRF levels in HPB were measured by radioimmunoassay...

...Sarah Nicholson; Megan Holmes; Winardi Sutanto; Susannah Watts; Elizabeth Campbell; Brian Gillham; Mortyn Jones Female rats were treated with two intraventricular injections each of 350 µg 6-hydroxydopamine (6-OHDA) and used for experiment 4 or 14 days later. The response to laparotomy, as assessed...

... of the PVN noradrenergic terminal plexus by local application of the neurotoxin 6-hydroxydopamine 1 day prior to electrophysiological experiments abolished the effects of both Al and A2 stimulation. These findings indicate that noradrenergic afferents can exert a facilitatory influence on the activity...

... injections of the selective neurotoxins, 5,7-dihydroxytryptamine and 6-hydroxydopamine, into the mediobasal hypothalamus, the medial preoptic area, the amygdala, and the medial forebrain bundle. Serum levels of luteinizing hormone (LH), follicle-stimulating hormone, testosterone, pituitary follicle...

...R.D. Stith; R.J. Person Dogs were bilaterally adrenalectomized (Adx) or sham adrenalectomized 2 weeks after the administration·6-hydroxydopamine (6-OHDA) or saline-ascorbic acid vehicle directly into the third ventricle (3V). Hypothalamic and hippocampal cytosols were assayed in vitro for high...

...J. Rabii; C. Ehlers; D. Clifton; C.H. Sawyer Repeated infusions of 6-hydroxydopamine (6-OHDA) into the third ventricle of the rabbit brain in dosages shown to depress hypothalamic norepinephrine (NE) by more than 80% failed to block the copulation-induced ovulatory surge of LH release from...

... and for intracerebroventricular (i.c.v.) injections. GH and Prl secretory profiles were obtained by radioimmunoassay of blood samples drawn every 15 min for a 6-hour period. Hormone profiles were obtained before, 1 day (day 1) and 7 days (day 7) after i.c.v. injection of 6-hydroxydopamine (6-OHDA, total dose 600 μg). GH...

...-day-old pups subjected to central sympathectomy by 6-hydroxydopamine (6-OHDA, 60 µ g/10 µ l by intraventricular route) 1 week before; in these animals, TRH was ineffective in releasing PRL even at the greatest dose level (1.5 µ g/100 g b.w.). In pups pretreated with 6-OHDA, the GH-lowering effect...

...M.T. Hyyppä; D.P. Cardinali; R.J. Wurtman Male rats treated postnatally witha single intraventricular injection of 10 µg of 6-hydroxydopamine (6-OHDA) exhibited a sig-nificant increase in pineal hydroxyindole-O-methyl transferase (HIOMT) activity on the 30th day of life; female animals failed...

... in the function of brain dopamine, there are also asymmetries in the peripheral response to experimental hemi-parkinsonism, performed by means of lesions of the nigrostriatal system with 6-hydroxydopamine (6-OHDA) injections into the left or right hemisphere. Therefore, it is speculated that the neuroendocrine...