Nuclear or total tissue binding of 3H-estradiol (3H-E2) was measured 1 h after i.v. injection in adult male and female rats gonadectomized 2 days, 1 week or 3 months earlier. Diethylstilbestrol- (DES) blockable nuclear 3H-E2 binding was highest in the anterior pituitary (PIT), followed by the preoptic area-anterior hypothalamus (POA-AH) and median eminence-basal hypothalamus (ME-BH), the amygdala (AMYG), dorsal hypothalamus (DH) and prehypothalamic area (PHA) and cerebral cortex (CX). DES administration reduced total tissue 3H-E2 uptake in the uterus of all groups, in the neurohypophysis (NH) of most groups and in the pineal of animals gonadectomized for 3 months. In both males and females, nuclear uptake of 3H-E2 was about 1.4 times higher 3 months after gonadectomy than 2 days after gonadectomy in the PIT and all brain areas except the ME-BH, where binding had approximately doubled during the same time period. Total 3H-E2 binding per organ in the NH was higher 3 months than 1 week or 2 days after gonadectomy in females. Nuclear 3H-E2 binding in the PIT was higher in males than in females 1 week after gonadectomy. There were no other sex differences in nuclear or total tissue 3H-E2 binding. The results suggest that the decrease in estradiol (E2) sensitivity of neuroendocrine mechanisms involved in gonadotropin secretion and reproductive behavior following prolonged absence of the gonads is not due to a decline in the ability of the PIT and several brain areas to bind E2, as measured 1 h after 3H-E2 injection.

Keywords:
3H-estradiol, Anterior pituitary, Gonadectomy, Hypothalamus, Amygdala, Pineal, Neurohypophysis, Estradiol receptor
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© 1976 S. Karger AG, Basel
1976
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