The pituitary glands of mature male rats were removed and either exposed to ice cold buffer solution or kept at room temperature for 5–15 min, and then incubated at 37°C. The levels of GH, LH and prolactin, released into the medium, were measured by specific radioimmunoassays. The pre-cooling of the pituitary glands augmented the basal release of these pituitary hormones during the first 1-hour period of incubation. Purified pig hypothalamic fractions were tested for GH-releasing hormone (GH-RH) and prolactin release-inhibiting factor (PIF) activity under the cooling or non-cooling conditions. PIF activity was demonstrated only when pituitaries were not exposed to cooling. GH-RH activity was not demonstrated under either condition in the fraction containing PIF.

Keywords:
GH, LH, Prolactin in vitro, Hypothalamic extract, PIF
This content is only available via PDF.
© 1974 S. Karger AG, Basel
1973
Copyright / Drug Dosage / Disclaimer
Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.
You do not currently have access to this content.