... in cultured human mesangial cells (MCs). Methods: We treated MCs with polyinosinic-polycytidylic acid (poly IC), an authentic double-stranded RNA, and analyzed the ISG15 expression by reverse transcription-polymerase chain reaction and western blotting. To examine the regulation of ISG15 expression, we...

...A. Krämer-Guth; M. Nauck; T. Quaschning; H. Pavenstädt; H. Wieland; P. Schollmeyer; C. Wanner Human low-density lipoprotein (LDL) is taken up by rat mesangial cells in a receptor-dependent manner, although lipoprotein metabolism and lipoprotein receptors differ substantially between rodents...

... to evaluate the effects of hydrocortisone and recombinant human lipocortin-1 on cultured human mesangial cells (CHMC), and the effects of anti-lipocortin-1 antibody on the hydrocortisone-induced inhibition of CHMC proliferation. The existence of specific binding sites for lipocortin-1 was also investigated...

...Pravin C. Singhal; Pratima Sharma; Krishna Reddy; Vibha Sanwal; Jagat Rawal; Nora Gibbons; Nicholas Franki Mesangial cell (MC) hyperplasia and accumulation of extracellular matrix are the predominant features of HIV-associated nephropathy (HIVAN). Since mice transgenic for HIV-1 genes show renal...

... cells infiltrating the interstitium. In areas of tubular atrophy there was increased staining for Cx43. Using reverse transcription-polymerase chain reaction we have also demonstrated that cultured human and rat mesangial cells and human proximal tubular cells express Cx43 messenger RNA. In summary, we...

... Macrophages seem to play an important role in the development of glomerulosclerosis. In the present study we evaluated the effect of morphine, an active metabolite of heroin, on the migration of monocytes across a filter in a modified Boyden chamber. Morphine-mesangial cell interaction enhanced (p...

...Gerald C. Groggel; M. Laurette Hughes Heparan sulfate, a glycosaminoglycan which is a component of the glomerular basement membrane and the mesangial matrix, has been demonstrated to inhibit mesangial cell growth. The present studies investigated the effects of heparan sulfate on extracellular...

... on the extracellular matrix production by cultured rat mesangial cells (CRMC). Subconfluent CRMC monolayers were grown with 10% oxygen (hypoxia) and 20% oxygen (control) for 1, 3, and 5 days. The production of type IV collagen (CIV), fibronectin (FN), and laminin (LN) by CRMC was evaluated by flow cytometry...

...Stefan Heidenreich; Martin Tepel; Detlef Lang; Karl-Heinz Rahn; Walter Zidek In this study, we compared the cellular functions of cultured glomerular mesangial cells (MC) from spontaneously hypertensive rats (SHR) and from normotensive Wistar-Kyoto rats (WKY) in response to the growth factors...

...Andrea L. Zuckerman; Kurt H. Stenzel; Manikkam Suthanthiran; Harry Holthofer; Detlef Schlondorff This paper investigates factors involved in mesangial cell (MC) proliferation by focussing on the proliferative effects of phorbol myristate acetate (PMA) on mouse MC in culture in comparison to those...

...Marc J. Sadovnic; Jessica Brand-Elnaggar; Kline Bolton Experimental autoimmune glomerulonephritis (EAG) in chickens appears to be mediated by cellular immunity and is associated with mesangial proliferation. We have developed techniques for the culture of chicken mesangial cells to study factors...

... by the contractile state of the cells of the mesangium. CS enhances contractions of mesangial cells elicited with angiotensin II. By way of a lowered ultrafiltration coefficient this effect of CS may be partly responsible for its acute nephrotoxicity. We have therefore examined the effect of compounds that may...

...Koichi Matsumoto; Michinobu Hatano The ability of immune complexes (IC) derived from rat renal tubular epithelial (RTE) antigen to trigger the production of interleukin-1 (IL-1) was investigated. When cultured rat mesangial cells (MC) were activated by the IC, substantial amounts of IL-1 could...

...Z. Averbukh; S. Berman; J. Weissgarten; M. Cohn; A. Golik; N. Cohen; D. Modai Rat kidney mesangial cells were procured from 5 sham-operated and 10 previously uninephrectomized rats. They were grown in cell culture medium supplemented with 20% fetal calf serum. Cell free conditioned medium...

...P.C. Singhal; R.M. Hays Mesangial cells are contractile and are believed to play a role in the regulation of glomerular filtration. Actin filaments constitute an integral part of the cytoskeleton of these cells. To visualize actin filaments in both living and fixed acetone-extracted (nonliving...

... microscopy and time-lapse cinemicroscopy. The pattern of growth was the same for all the species studied. In all species, three cell populations have been identified with the features of epithelial cells, mesangial cells and macrophages, although the latter population is only rarely observed. The morphology...

... tubular epithelial cells (EPC), mesangial cells (MC) and glomerular endothelial cells (GEDC) as well as endothelial cells (EDC) and myofibroblasts (MF) from cattle were isolated to measure the proliferation and protein synthesis in the presence of individual and combined cytokines/growth factors in cell...

... NO on pro-inflammatory cytokine-induced MCP-1 expression in human mesangial cells and its signal transduction pathway. Cells were pretreated with NO donors such as 3-morpholino-sydnonimine (SIN-1) or nitroprusside, and then stimulated with tumor necrosis factor-α (TNF-α) or interleukin-1β (IL-1β). MCP-1...

... obtained in this study indicate increased mesangial expression of 5′-nucleotidase in non-proliferative form of glomerulonephritis consistent to a role of mesangial cells in inflammatory processes. References 1. Stefanović V, Savić V, Vlahović P, Ardaillou N, Ardaillou R: Ecto-5′-nucleotidase...

... mesangial cell ectoenzymes: 5′-nucleotidase, Mg 2+ -ATPase and Ca 2+ -ATPase. Ecto-5′-nucleotidase activity was significantly increased after 48 h treatment with spermine and spermidine. Mg 2+ -ATPase was increased only after treatment with spermidine; however, Ca 2+ -ATPase was significantly increased...