... fibrosis (RIF) through regulating M1 macrophage (M1) polarization. Method: Nine-week-old mice underwent unilateral ureteral obstruction (UUO) establishment. Macrophages were induced toward M1 polarization using lipopolysaccharide (LPS) in vitro and cocultured with fibroblasts to examine how M1 polarization...

...Yining Li; Weihong Yu; Mingyue He; Fang Yuan Objectives: The aim of this study was to explore the effects of M1/M2 macrophages on the mRNA expression profile of glomerular endothelial cells (GECs) treated with advanced glycosylation end products (AGEs). Methods: We cocultured M1/M2 macrophages...

... of immunoreactivity of cross-linked FRA (XL-FRA) using the monoclonal antibody DD3B6/22 after plasmin exposure was evaluated to assess intraglomerular deposition of intact XFb. Also, intraglomerular invasion of macrophages was detected using the monoclonal antibody KP1 against CD68. Sixteen of a total of 37 specimens...

...Motoshi Hattori; Katsumi Ito; Hiroshi Kawaguchi; Yutaka Yamaguchi To investigate the potential roles of oxidized lipoproteins and glomerular macrophages in the pathogenesis of lipid-induced glomerular injury, we examined the effects of administration of probucol on the development of renal injury...

...Nobuaki Yoshimura; Seiya Arima; Mahito Nakayama; Tatsuo Sato; Kiyoshi Takahashi Morphological and immunohistochemical studies using the anti-macrophage monoclonal antibody RbM2 were performed in cholesterol-fed rabbits. From the beginning of the experiment, the levels of lipoproteins showed...

... glomerular lesions within 4-8 weeks after initiation of the diet. The most striking glomerular change observed in this model was the marked accumulation of numerous lipid-filled foam cells, with a surface marker that identified them as macrophages, within the mesangial regions which could have been involved...

... ± 0.38 vs. 1.42 ± 0.35 g, p < 0.02). There was a significant increase in glomerular leukocytes on the cholesterol diet (4.10 ± 0.44 vs. 2.86 ± 0.25 OX-1 positive cells/10 4 μ 2 , p < 0.05). Mesangial foam cells, derived from macrophages, were associated with adhesions to Bowman’s capsule...

...Klaas Vlaanderen; HarryJ. Bos; Carola W.H. de Fijter; Liem P. Oe; Jan van der Meulen; Henri A. Verbrugh; Robert H.J. Beelen The effect of different intraperitoneal dwell times on the phagocytic capacity of the effluent-derived macrophages in 6 peritoneal dialysis patients was studied. The number...

... microscopy and time-lapse cinemicroscopy. The pattern of growth was the same for all the species studied. In all species, three cell populations have been identified with the features of epithelial cells, mesangial cells and macrophages, although the latter population is only rarely observed. The morphology...

... with inosine monophosphate dehydrogenase. Recent studies have demonstrated that mizoribine improves renal tubulointerstitial fibrosis in the rat model of unilateral ureteral obstruction (UUO) by inhibiting the infiltration of macrophages. We, therefore, examined the dose dependency of the suppressive effect...

... biopsy. The cells were analyzed with a three-layer indirect immunoperoxidase method using monoclonal antibodies against leukocyte common antigen, T cells, B cells, and monocytes/macrophages (Mo/Mφ). Results: Renal function tended to deteriorate during the final follow-up period in group MN+GS...