... nephropathy (passive Heymann nephritis model) and mice with experimental FSGS (anti-glomerular antibody model) following the onset of proteinuria. Immunohistochemistry staining of PAX2 (parietal epithelial cell marker), WT-1 (podocyte cell marker), and Ki-67 (proliferation marker) were performed on kidney...

... progressive worsening of podocyte and glomerular damage in both male and female Rab3A KO. Though glomerular lesions, reminiscent of human diabetic nephropathy, were more severe in male mice, overt proteinuria and renal damage were also evident in female mice. The in vitro analysis of Rab3A WT and KO podocytes...

... weight BZ i.v., Janssen-Cilag, Germany) for a period of 10 and 8.5 months. In the first group, BZ treatment was started before the onset of proteinuria at the age of 18 weeks (n = 10). The second group was treated with PBS at the age of 18 weeks followed by BZ treatment at the age of 24 weeks when...

...Jeanette Eyre; James O. Burton; Moin A. Saleem; Peter W. Mathieson; Peter S. Topham; Nigel J. Brunskill Background/Aims: Proteinuria is associated with cardiovascular and chronic kidney disease. Microparticles (MPs) are bioactive vesicles shed from activated cells and also linked to cardiovascular...

..., instructions or products referred to in the content or advertisements. 2010 Adc1 Adenylate cyclase Albuminuria Glomerular podocytes Proteinuria NIH Podocytes play a critical role in the maintenance of the normal glomerular filtration barrier [ 1 ]. This is highlighted...

... CTGF expression was mainly localized in podocytes. Accordingly, elevation of urinary, plasma, and tissue CTGF in UNX-SHR coincided and correlated with proteinuria, glomerulosclerosis, and tubulointerstitial fibrosis. Thirty-two weeks after uninephrectomy, mean glomerular CTGF mRNA expression...

... of TF (TF CT–/– mice) were used to study its role in physiological albuminuria and pathological proteinuria following induction of glomerulonephritis (GN). Results: Absence of the cytoplasmic domain of TF was associated with increased albuminuria, podocyte effacement, reduced podocyte numbers...

.... The model should be helpful in investigating pathophysiologic mechanisms and exploring therapeutic interventions for myeloma kidney and other LC-associated renal disorders. We first tested the effects of LCs in intact mice in the LD experiment. Treatment with LCs led to LC proteinuria, confirmed as ĸ...

...Fredrik Dunér; Karin Lindström; Kjell Hultenby; Jenny Hulkko; Jaakko Patrakka; Karl Tryggvason; Börje Haraldsson; Annika Wernerson; Erna Pettersson Background/Aims: It is still unclear what happens in the glomerulus when proteinuria starts. Using puromycin aminonucleoside nephrosis (PAN) rats, we...

... ameliorates proteinuria in puromycin aminonucleoside (PAN) nephropathy, the effect of MZR on podocytes has not been clarified. In this study, we determined whether MZR directly prevents PAN-induced podocyte injury. Methods: Rats were intravenously injected once on day 0 with 100 mg/kg of PAN and received...

...Masaru Motojima; Taiji Matsusaka; Valentina Kon; Iekuni Ichikawa Background: Composition of nonselective proteinuria includes several endogenous ligands of Toll-like receptors (TLRs) not normally present in Bowman’s space, thus raising the possibility that TLRs are involved in proteinuria-mediated...

..., instructions or products referred to in the content or advertisements. 2009 Chemokine Glomeruli In situ hybridization Proteinuria Puromycin aminonucleoside nephrosis Western blotting A chemokine fractalkine (FKN/CX3CL1) is the only one member of the CX 3 C chemokine family characterized...

... by subepithelial immune deposits, podocyte foot processes effacement and massive proteinuria beginning 4 days following disease induction. Although single genes involved in PHN have been studied, no whole genome-wide expression analysis of kidney tissue has been performed. Methods: Microarray analysis...

... to day 28. The reduction in VEGF and phosphorylated nephrin was not a result of podocyte loss. The intervention of prednisone and lisinopril evidently reduced proteinuria, effectively attenuated the severe lesions of podocyte foot processes, and restored the reduction in VEGF and nephrin phosphorylation...

... and immunohistochemical staining. Results: Treated animals showed reduction in serum AGEs (p < 0.01), proteinuria (p < 0.01) and systolic blood pressure (p < 0.01). GSPE reduced the expression of RAGE (p < 0.01) and CTGF (p < 0.01) in the kidney, which were contributing to reversal of extracellular matrix...

... that disturbances of podocyte biology contribute to proteinuria in women with preeclampsia (PE). Methods: A human podocyte cell line was stimulated with serum from women with PE (patients) and healthy pregnant women (controls); the main changes in 3 important podocyte proteins: podocin, CD2AP and actin were...

...Richard J. Baines; Nigel J. Brunskill Proteinuria is associated with progressive chronic kidney disease and poor cardiovascular outcomes. Exposure of proximal tubular epithelial cells to excess proteins leads to the development of proteinuric nephropathy with tubular atrophy, interstitial...

...Shigetaka Yoshida; Miki Nagase; Shigeru Shibata; Toshiro Fujita Background: Proteinuria is a well-established exacerbating factor in chronic kidney disease. Although the mechanisms of albumin-induced tubulointerstitial damage have been extensively studied, the influence of proteinuria on podocytes...

...Young-Il Jo; Huifang Cheng; Suwan Wang; Gilbert W. Moeckel; Raymond C. Harris Previous studies from our own group and others have demonstrated that cyclooxygenase-2 (COX-2) inhibitors could reduce proteinuria in some experimental models of progressive renal disease. To investigate a possible role...

... injury only when combined with ACE inhibitor. We investigated whether in a two-kidney diabetic model the time at which treatment started predicted the response to TGFβ antagonist. Methods: 27 weeks after streptozotocin injection, animals had mild proteinuria and were randomized to receive irrelevant...