... defense reactions against RNA viruses. The expression and the role of ISG20 in mesangial cells have not been reported. Methods: Normal human mesangial cells were cultured and treated with polyinosinic-polycytidylic acid (poly (I:C)), an authentic double-stranded RNA which mimics viral infection to cells...

...Hassan Khammas; Timothy Bowen; John David Williams; Aled Owain Phillips; Robert Steadman; John Martin Background: ADAM15 is a membrane-bound member of the adamalysin family that is up-regulated in areas of tissue remodelling. Previous studies have demonstrated the role of ADAM15 in mesangial cell...

...Christopher J. Mulgrew; Andrea Cove-Smith; Linda M. McLatchie; Gavin Brooks; Michael J. Shattock; Bruce M. Hendry Background: Aberrant glomerular mesangial cell (MC) proliferation is a common finding in renal diseases. T-type calcium channels (T-CaCN) play an important role in the proliferation...

...Quancheng Feng; Songming Huang; Aihua Zhang; Qiuxia Chen; Xirong Guo; Ronghua Chen; Tianxin Yang Aims: To investigate the effects of Y-box protein 1 (YB-1) on the mitogen-activated protein kinase pathway to ascertain which signaling pathway is involved in YB-1-induced mesangial cell (MC...

... glomerulonephritis was induced in male Wistar rats with a monoclonal anti-rat Thy-1.1 antibody (OX-7) with rats randomized to receive either tranilast 400 mg/kg/day or vehicle control. Collagen synthesis and proliferation of cultured mesangial cells following incubation with PDGF (50 ng/ml) and tranilast (10–100 µ M...

...Shuji Iwatsubo; Shouichi Fujimoto; Michitaka Matsumoto; Yuji Sato; Seiichiro Hara; Kazuo Kitamura; Tanenao Eto Background/Aims: Adrenomedullin (AM) has anti-proliferative and apoptotic effects on mesangial cells (MCs). Both effects play an important role in the progression of glomerulonephritis (GN...

...Aditi A. Kapasi; Saijun Fan; Pravin C. Singhal HIV-1 gp120 protein has been shown to promote mesangial cell (MC) injury. Scatter factor (SF) is a growth factor that plays a reparative role in various experimental models of renal lesions. We hypothesize that SF protects MC against HIV-1 gp120...

... Glomerulus Mesangial cells Extracellular matrix Microfibrillar proteins Transforming growth factor-β Anti-Thy1.1 nephritis Latent transforming growth factor-β-binding protein 1 (LTBP-1) regulates secretion and activation of transforming growth factor-β (TGF-β) isoforms [ 1 ] and also...

... of CRP on the nuclear factor-ĸB (NF-ĸB) activation and monocyte chemoattractant protein-1 (MCP-1) gene expression in human mesangial cells and also examined whether intracellular calcium and reactive oxygen species (ROS) were involved in the CRP- induced NF-ĸB activation. Methods: NF-ĸB binding activity...

... Mesangial cells Regulation of signal transduction and gene expression is a multifaceted process involving ligands, receptors and second messengers that trigger cascades of protein kinases and phosphatases and propagate the signal to the nucleus to alter gene expression. Reduction-oxidation (redox...

... and specifically could underlie growth hormone (GH)-induced glomerulosclerosis. The present study investigates the role of GH in the regulation of LDL-r and HMG-CoA reductase mRNA expression in mesangial cells. Mouse mesangial cells were equilibrated in a medium containing 5% lipoprotein-deprived serum (LPDS...

... and development. Increased expression of gremlin has recently been demonstrated in several models of diabetic nephropathy. Gremlin arrests the cell cycle in mesangial cells and has also been shown to be upregulated in transdifferentiated renal proximal tubular cells. This review summarizes emerging evidence...

... dysfunction of GECs and mesangial cells can lead to persistent glomerular perturbations with prolonged phenotypic change of mesangial cells, resulting in end-stage renal deficiency. GEC damage during the acute nephritic phase contributes to the progression of irreversible renal disease. 27 6 2002...

... in lupus nephritis as are changes in mesangial matrix. Since ON competitively bind to glomerular mesangial cells (MC) in a receptor-like fashion, the purpose of our study was to investigate what effects ON have on MC matrix and proliferation. Methods: Rat and mouse MCs grown with ON or DNA for 1 week were...

...), a component of the IGF system, has been shown to induce apoptosis in cancer cell lines. However, if IGFBP-3 has similar effects in human mesangial cells (HMC) remains unknown. The purpose of this study was to examine the expression of IGFBP-3 and its possible effect on the induction of apoptosis in HMC during...

... of this study was to investigate whether there is an altered cultured mesangial cell (MC) phenotype in RF/J mice associated with these glomerular changes. Methods: The nature of cultured MCs from RF/J mice in the proliferative response to platelet-derived growth factor (PDGF) BB was compared with that of normal...

... whether the transcription factor, nuclear factor ĸB (NFĸB), mediated the expression of these genes involved with the inflammatory response of mesangial cells by using transcription factor decoy oligodeoxynucleotides (ODN) to block NFĸB binding to the promoter site of its target genes. We hypothesized...

... coinciding with the very early induction of mesangial injury. The present review is focused on apoptosis in relation to this model, and discusses recent findings concerning direct involvement of triggering of Thy-1 as well as deposition of terminal complement complexes in the induction of mesangial cell...

... TSHR transcript in the renal cortex of transgenic animals. TSHR transcript was also detected in mouse mesangial cells in vitro which responded to TSH with significant increases in the formation of three-dimensional hillhocks. Polymerase chain reaction also confirmed the presence of Tg transcript...

... be dissociated from a rise in IκB-α protein. We investigated the role of IκB-α in mesangial cells using a phosphorothioate antisense oligonucleotide directed against the translational start point of IκB-α. If IκB-α does function as a negative feedback inhibitor in these cells, then reducing IκB-α levels should...