...Gleb Martovetsky; Sanjay K. Nigam Background: With the rate of kidney disease on the rise, and a serious imbalance between the number of patients requiring a kidney transplant and the number of available donor kidneys, it is becoming increasingly important to develop alternative strategies...

...Ravi Katari; Andrea Peloso; Joao Paulo Zambon; Shay Soker; Robert J. Stratta; Anthony Atala; Giuseppe Orlando Background: In 2012, about 16,487 people received kidney transplants in the USA whereas 95,022 candidates were on the waiting list at the end of the year. Moreover, more than 2,600 kidneys...

.../progenitor cells, the embryonic zone of neonatal rabbit kidney was fixed either with glutaraldehyde (GA) or in a combination with cupromeronic blue, ruthenium red or tannic acid. Transmission electron microscopy was then performed on exactly orientated sections. Results: Conventional fixation with GA...

...L.L. Huang; D.J. Nikolic-Paterson; F.Y. Ma; G.H. Tesch Background/Aims: The mineralocorticoid hormone, aldosterone, has pro-fibrotic properties which can cause kidney damage. The severity of kidney interstitial fibrosis is dependent on the accumulation of fibroblasts, which result largely from...

...Kenneth A. Walker; Georgina Caruana; John F. Bertram; Kerry J. McInnes 17β-Estradiol, the most potent circulating estrogen, has been shown to greatly impact on the development and formation of tissues of the urogenital tract. The adult kidney has previously been shown to be highly responsive to 17β...

.... Heterologous expression studies confirm that both Rhbg and Rhcg transport ammonia and the ammonia analog, methylammonia, and that their affinity for ammonia is similar to physiologically relevant ammonia concentrations present in the kidney [ 9, 10, 11, 12 ]. Transport studies using cultured mouse collecting...

.... The kidney contains quite a number of different cell types, the function of many of which impacts that of other renal cells. To avoid these limitations conditional knockout strategies have been designed. As one important part of this system we describe the development of a mouse line expressing the tamoxifen...

... morphine or hydromorphone in clinically relevant doses, or PBS (controls). Renal function was assessed by blood and urine chemistry as well as by measuring mean arterial pressure (MAP) and kidney perfusion. Pathological changes in the kidneys were examined by routine histology. Molecular changes were...

... from any ideas, methods, instructions or products referred to in the content or advertisements. 2006 Heme oxygenase Bilirubin Biliverdin Carbon monoxide Kidney Ischemia/reperfusion Renal ischemia is a consequence of arterial occlusion, shock and organ transplantation...

... Kidney The kidney, which is one of the most transplanted organs, may be subjected to ischemia-reperfusion (I-R) injury leading to delayed graft function as well as short- and long-term complications. After renal transplantation, acute tubular necrosis is observed in approximately 19–50...

... or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements. 2005 Cell cycle Kidney Glomerulus Proliferation Podocyte Mesangial cell Progressive glomerulosclerosis Hypertrophy Apoptosis Glomerular diseases are a leading cause...

... in rats with passive Heymann nephritis (PHN) of CsA and mycophenolate mofetil (MMF). Methods: PHN was induced in female Wistar rats. Two treatment groups consisting of 8 rats each received either 25 mg of CsA or 25 mg of MMF/kg body weight/day and were compared with untreated controls. Kidney function...

...Anil Karihaloo; Christian Nickel; Lloyd G. Cantley The phenomenon of branching morphogenesis is a fundamental process critical for development of several tubular organs including lung, mammary gland, and kidney. In the case of kidney, the ureteric bud (UB) that extends out from a pre-existing...

... with chronic renal failure. The aim of the study was to investigate CD146 expression on biopsies of normal kidney and nephropathies. Methods: We did an immunohistochemical analysis of 10 normal renal tissues and 126 patients with nephropathies. Results: The mean age of the patients was 47.5 ± 18 years with 65...

...). An inverse correlation between the expression of COX-2 and α v integrins in rats with PHN was demonstrated. Conclusions: It is suggested that COX-2-derived prostaglandins suppress the expression of α v integrins. This implies a previously unknown role for COX-2 in chronic inflammation in the kidney. Urine...

... or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements. 2004 Osteopontin Hypoxia ATP depletion Renal proximal tubular cells Kidney Ischemic acute renal failure is associated with the induction of osteopontin (OPN) [ 1, 2 ]; its...

... physiological and pathophysiological importance. In this study, in situ RT-PCR was utilized to detect tubular expression of prepro-ET-1 mRNA in normal rat kidney. Methods: Kidneys were taken from normal Sprague-Dawley rats weighing approximately 200 g. In situ RT-PCR was carried out using the preparations...

... base, 192 m M glycine, and 0.1% sodium dodecylsulfate (SDS). Upper running buffer was 2× solution of the lower buffer. The system was run with maximal 500 V and 20,000 MW per gel. Proteomics Proteome Kidney Biomarker L-myc Ezrin GAP-43 2-D PAGE 2-D Western blot Posttranslational...

... the RiboAmp ® amplification kit in the setting of cDNA microarrays. We isolated mRNA from a human kidney cell line (HK-2; ATCC) and from Universal Human Reference RNA (STR; Stratagene). After performing one and two rounds of linear RNA amplification, respectively, the amplified RNAs were co-hybridized to cDNA...

... and distribution of integrins in clear cells of diabetic animals must have defined roles in the development of the renal tubulopathy. For each animal group, tissues from 3 rats were used and pooled. The kidneys were removed and placed in ice-cold PBS (pH 7.4). The area comprising the outer stripe of the outer...