...) proliferation. Methods: The rat MC line HBZY-1 was transfected with pcDNA3.1-YB-1. Cell proliferation was determined by [ 3 H]thymidine incorporation, cell counting and cell-cycle analysis . The expression of cyclins was examined by real-time RT-PCR and immunoblotting analysis. Phosphorylation of c-Raf, MEK1/2...

...Kengo Furuichi; Takashi Wada; Shinji Kitajikma; Tadasi Toyama; Toshiya Okumura; Akinori Hara; Hiroshi Kawachi; Fujio Shimizu; Takeshi Sugaya; Naofumi Mukaida; Shosaku Narumi; Kouji Matsushima; Shuichi Kaneko Although renal tubular cell proliferation after acute tubular necrosis is an important...

... ) was determined by 3 H-proline and 3 H-thymidine incorporation, respectively. Results: Tranilast treatment resulted in a significant reduction in mesangial cell proliferation, macrophage infiltration, activated (α-smooth muscle actin positive) mesangial cells, glomerular type IV collagen deposition...

... (αMCS) ( A ) and control serum ( B ). A single cell in panel A is labeled (arrowhead), indicating this is a cell undergoing apoptosis. Sections were stained for expression of proliferating cell nuclear antigen (PCNA) as an index of cell proliferation at day 4, following administration of mesangial...

... restriction of dietary sodium content (0.25 vs. 0.50% sodium w/w) affected AP-1-DNA binding and hyperplasia in the remnant kidney after nephron reduction (70% nephrectomy). Cell proliferation, evaluated by PCNA immunostaining, increased progressively from day 7 to day 60 in glomeruli, proximal and distal...

... process fusion Cytoskeleton Cell proliferation The podocytes or visceral glomerular epithelial cells are injured in many forms of glomerular disease. The earliest and commonest consequence of podocyte injury includes the development of proteinuria, which reflects structural changes...