Abstract
Capillary electrophoresis has recently emerged as a powerful technique for separating components in biological samples. A family of separation methods capable of handling a diverse range of samples has been developed, the sample volumes required are very small and a wide range of specialised detectors can be employed. This review examines some methods with particular application to the analysis of urine and tubular fluid samples and references relevant applications.
References
1.
Shihabi ZK, Friedberg MA: Analysis of small molecules for clinical diagnosis by capillary electrophoresis. Electrophoresis 1997;18:1724–1732.
2.
Lunte SM, O’Shea TJ: Pharmaceutical and biochemical applications of capillary electrophoresis with electrochemical detection. Electrophoresis 1994;15:79–86.
3.
Landers JP: Clinical capillary electrophoresis. Clin Chem 1995;41:495–509.
4.
Chen FT: Capillary electrophoresis – A new clinical tool. Clin Chem 1991;37:14–19.
5.
Shihabi ZK: Clinical applications of capillary electrophoresis. Ann Clin Lab Sci 1992;22:398–405.
6.
Deyl Z, Tagliaro F, Miksik I: Biomedical applications for capillary electrophoresis. J Chromatogr B Biomed Appl 1994;656:3–27.
7.
Jenkins MA, Guerin MD: Capillary electrophoresis as a clinical tool. J Chromatogr B Biomed Appl 1996;682:23–34.
8.
Wildman BJ, Jackson PE, Jones WR, Alden PG: Analysis of anion constituents of urine by inorganic capillary electrophoresis. J Chromatogr 1991;546:459–466.
9.
Holmes RP: Measurement of urinary oxalate and citrate by capillary electrophoresis and indirect ultraviolet absorbance. Clin Chem 1995;41:1297–1301.
10.
Sevcik J, Adam T, Sazel V: A rapid and simple screening method for detection of orotic aciduria by capillary zone electrophoresis. Clin Chem Acta 1997;259:73–81.
11.
Franke DR, Nuttall KL: Orotic acid in clinical urine specimens by capillary zone electrophoresis using polyvinylalcohol coated capillaries. J Capillary Electrophor 1996;3:309–312.
12.
Meulemans A, Delsenne F: Measurement of nitrite and nitrate levels in biological samples by capillary electrophoresis. J Chromatogr B Biomed Appl 1994;660:401–404.
13.
Friedberg MA, Hinsdale ME, Shihabi ZK: Analysis of nitrate in biological fluids by capillary electrophoresis. J Chromatogr A 1997;781:491–496.
14.
Gilman SD, Ewing AG: Analysis of single cells by capillary electrophoresis with on-column derivatization and laser induced fluorescence detection. Anal Chem 1995;67:58–64.1
15.
Tong W, Yeung ES: On-column monitoring of secretion of catecholamines from single bovine adrenal chromaffin cells by capillary electrophoresis. J Neurosci Methods 1997;76:193–201.
16.
Zhu R, Kok WT: Determination of catecholamines and related compounds by capillary electrophoresis with post column terbium complexation and sensitized luminescence detection. Anal Chem 1997;69:4010–4016.
17.
Robert F, Bert L, Denoroy L, Renaud B: Capillary zone electrophoresis with laser induced fluorescence detection for the determination of nanomolar concentrations of noradrenaline and dopamine: Application to brain microdialysate analysis. Anal Chem 1995;67:1838–1844.
18.
Bert L, Robert F, Denoroy L, Renaud B: High speed separations of subnanomolar concentrations of noradrenaline and dopamine using capillary zone electrophoresis with laser induced fluorescence detection. Electrophoresis 1996;17:523–525.
19.
Chang HT, Yeung ES: Determinations of catecholamines in single adrenal medullary cells by capillary electrophoresis and laser induced fluorescence. Anal Chem 1995;67:1079–1083.
20.
Chan KC, Muschik GM, Issaq HJ: Separation of tryptophan and related indoles by micellar electrokinetic chromatography with KrF laser induced fluorescence. J Chromatogr A 1995;718:203–210.
21.
Mank AJ, Yeung ES: Diode laser-induced fluorescence detection in capillary electrophoresis after pre-column derivitization of amino acids and small peptides. J Chromatogr A 1995;708:309–321.
22.
Jellum E, Thorsrud AK, Time E: Capillary electrophoresis for diagnosis and studies of human disease, particularly metabolic disorders. J Chromatogr 1991;559:455–465.
23.
Jin W, Wang Y: Determination of cysteine by capillary zone electrophoresis with end column amperometric detection at a gold/mercury amalgam microelectrode without deoxygenation. J Chromatogr A 1997;769:307–314.
24.
Zhou J, Lunte SM: Direct determination of amino acids by capillary electrophoresis/electrochemistry using a copper microelectrode and zwitterionic buffers. Electrophoresis 1995;16:498–503.
25.
Gordon MJ, Huang X, Pentoney SL, Zare RN: Capillary electrophoresis. Science 1988;242:224–228.
26.
Weber PL, Malis M, Palmer SD, Klein TL, Lunte SM: Capillary zone electrophoresis separation of tryptophan and its metabolites including quinolinic acid. J Chromatogr B Biomed Appl 1997;697:263–268.
27.
Sevcik J, Adam T, Mazacova H: A fast and simple screening method for detection of 2,8-dihydroxyadenine urolithiasis by capillary zone electrophoresis. Clin Chem Acta 1996;245:85–92.
28.
Jariego CM, Hernanz A: Determination of organic acids by capillary electrophoresis in screening of organic acidurias. Clin Chem 1996;42:477–478.
29.
Messana I, Rossetti DV, Casstano L, Misiti F, Giardina B, Castagnola M: Peptide analysis by capillary (zone) electrophoresis. J Chromatogr B Biomed Appl 1997;699:149–171.
30.
Lim BC, Sim MK: Determination of angiotensins by capillary electrophoresis. J Chromatogr B Biomed Appl 1994;655:127–131.
31.
Koezuka K, Ozaki H, Matsubara N, Terabe S: Separation and detection of closely related peptides by micellar electrokinetic chromatography coupled with electrospray ionization mass spectrometry using the partial filling technique. J Chromatogr B Biomed Appl 1997;689:3–11.
32.
Matsubara N, Koezuka K, Terabe S: Separation of eleven angiotensin analogues by capillary electrophoresis with a non-ionic surfactant. Electrophoresis 1995;16:580–583.
33.
Chen FT: Characterisation of protease-catalyzed hydrolysis of cyanine-labelled angiotensin using capillary electrophoresis with laser induced fluorescence. Anal Biochem 1995;225:341–345.
34.
Sim MK, Lim BC: Determination of aminopeptidase X activity in tissues of normo- and hypertensive rats by capillary electrophoresis. J Chromatogr B Biomed Appl 1997;697:259–262.
35.
Jenkins MA: Clinical application of capillary electrophoresis to unconcentrated human urine proteins. Electrophoresis 1997;18:1842–1846.
36.
Friedberg MA, Shihabi ZK: Urine protein analysis by capillary electrophoresis. Electrophoresis 1997;18:1836–1841.
37.
Jenkins MA, O’Leary TD, Guerin MD: Identification and quantification of human urine proteins by capillary electrophoresis: J Chromatogr B Biomed Appl 1994;662:108–112.
38.
Oda RP, Clark R, Katzmann JA, Landers JP: Capillary electrophoresis as a clinical tool for the analysis of protein in serum and other body fluids. Electrophoresis 1997;18:1715–1723.
39.
Jellum E, Dollekamp H, Blessum C: Capillary electrophoresis for clinical problem solving: Analysis of urinary diagnostic metabolites and serum proteins. J Chromatogr B Biomed Appl 1996;683:55–65.
40.
Jellum E, Dollekamp H, Brunsuig A, Gislefoss R: Diagnostic applications of chromatography and capillary electrophoresis. J Chromatogr B Biomed Appl 1997;689:155–164.
41.
Presto Elgstoen KB, Jellum E: Capillary electrophoresis for the diagnosis of metabolic disease. Electrophoresis 1997;18:1857–1860.
42.
Petucci CJ, Kantes HL, Strein TG, Veening H: Capillary electrophoresis as a clinical tool. Determination of organic anions in normal and uraemic serum using photodiode-array detection. J Chromatogr B Biomed Appl 1995;668:241–251.
43.
Tran TC, Huq TA, Kantes HL, Crane JN, Strein TG: Determination of creatinine and other uremic toxins in human blood sera with micellar electrokinetic capillary electrophoresis. J Chromatogr B Biomed Appl 1997;690:35–42.
44.
Shirao MK, Suzuki S, Kobayashi J, Nakazawa H, Mochizuki E: Analysis of creatinine, vanilmandelic acid, homovanillic acid and uric acid in urine by micellar electrokinetic chromatography. J Chromatogr B Biomed Appl 1997;693:463–467.
45.
Shihabi ZK, Hinsdale ME, Bleyer AJ: Xanthine analysis in biological fluids by capillary electrophoresis. J Chromatogr B Biomed Appl 1995;669:163–169.
46.
Xu DK, Hua L, Li ZM, Chen HY: Identification and quantitative determination of uric acid in human urine and plasma by capillary electrophoresis with amperometric detection. J Chromatogr B Biomed Appl 1997;694:461–466.
47.
Transfiguracion JC, Dolman C, Eidelman DH, Lloyd DK: Determination of the inorganic ion composition of rat airway surface fluid by capillary electrophoresis: Direct sample injection to allow multiple analysis from nanolitre volumes. Anal Chem 1995;67:2937–2942.
48.
Friedberg M, Shihabi ZK: Analysis of urinary N-acetyl-beta-glucosamindase by capillary zone electrophoresis. J Chromatogr B Biomed Appl 1997;695:187–191.
49.
Bergert JH, Liedtke RR, Oda RP, Landers JP, Wilson DM: Development of a nonisotopic capillary electrophoresis based method for measuring glomerular filtration rate. Electrophoresis 1997;18:1827–1835.
50.
Wilson DM, Bergert JH, Larson TS, Liedtke RR: GFR determined by nonradiolabelled iothalamate using capillary electrophoresis. Am J Kidney Dis 1997;30:646–652.
51.
Rocco MV, Buckalew VM Jr, Moore LC, Shihabi ZK: Capillary electrophoresis for the determination of glomerular filtration rate using nonradioactive iohexol. Am J Kidney Dis 1996;28:173–177.
52.
Zanaboni G, Grimm R, Dyne KM, Rossi A, Cetta G, Iadarola P: The use of capillary electrophoresis for the analysis of imidodipeptides in urine prolidase-deficient patients. J Chromatogr B Biomed Appl 1997;694:461–466.
53.
Grimm R, Zanaboni G, Viglio S, Dyne K, Valli M, Cetta G, Iadorola P: Complete resolution of imidodipeptide mixtures in urine of prolidase-deficient patients using micellar electrokinetic chromatography. J Chromatogr A 1997;768:57–66.
54.
Zanaboni G, Grimm R, Dyne KM, Rossi A, Cetta G, Iadarola P: Use of capillary zone electrophoresis for analysis of imidodipeptides in urine of prolidase-deficient patients. J Chromatogr B Biomed Appl 1996;683:97–107.
55.
Gimm R, Zanaboni G, Viglio S, Dyne KM, Cetta G, Iadarola P: Effects of different surfactants on the separation by micellar electrokinetic chromatography of a complex mixture of dipeptides in urine of prolidase-deficient patients. J Chromatogr B Biomed Appl 1997;698:47–57.
56.
Wronski M: Separation of urinary thiols as tributyltinmercaptides and determination using capillary isotachophoresis. J Chromatogr B Biomed Appl 1996;676:29–34.
57.
Rickard EC, Towns JK: Applications of capillary zone electrophoresis to peptide mapping. Methods Enzymol 1996;271:237–264.
58.
Weinberger R, Sapp E, Moring S: Capillary electrophoresis of urinary porphyrins with absorbance and fluorescence detection. J Chromatogr 1990;516:271–285.
59.
Chiang SC, Li SF: Separation of porphyrins by capillary electrophoresis in fused silica and ethylene vinyl acetate copolymer capillaries with visible absorbance detection. Biomed Chromatogr 1997;11:366–370.
60.
Rao LU, Petersen JR, Bissell MG, Okorodudu AO, Mohammed AA: Specific determination of urinary free cortisol by solid phase microparticle extraction capillary electrophoresis with fused silica capillaries. Clin Chem 1997;43:1801–1803.
61.
Chan KC, Muschik GM, Issaq HJ: Separation of tryptophan and related indoles by micellar electrokinetic chromatography with KrF laser induced fluorescence. J Chromatogr A 1995;718:203–210.
62.
Shihabi ZK: Myoglobinuria detection by capillary electrophoresis. J Chromatogr B Biomed Appl 1995; 669:53–58.
1998
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