Background: Animal models of chronic renal failure have been widely used in the experimental nephrology laboratories. The most common technique used is the 5/6 reduction of renal mass, either by surgical resection or by infarction. Methods: In the present work, we describe a forgotten technique based in the ligation of the renal parenchyma in both renal poles. This technique combines the advantages of the resection model, like the reproducibility and homogeneity, with the ones of the infarction technique, like the absence of bleeding. Results: 8 weeks after the procedure, animals showed a decrease in creatinine clearance together with an increase in plasma creatinine. Furthermore, glomeruli of animals with 5/6 nephrectomy showed a marked hypertrophy, with a glomerular volume significantly higher than control animals. Serum levels of parathyroid hormone were also increased, consistent with the development of secondary hyperparathyroidism. Conclusions: We conclude that the present technique is a valid and improved tool for the study of chronic renal failure.

1.
Shimamura T, Morrison AB: Progressive glomerulosclerosis occurring in partial 5/6 nephrectomized rats. Am J Pathol 1975;79:95–106.
[PubMed]
2.
Purkerson ML, Hoffsten PE, Klahr S: Pathogenesis of glomerulopathy associated with renal infarction in rats. Kidney Int 1976;9:407–417.
[PubMed]
3.
Liu ZC, Chow KM, Chang TMS: Evaluation of two protocols of uremic rat model: partial nephrectomy and infarction. Renal Failure 2003;25:935–943.
[PubMed]
4.
Boudet J, Man NK, Pils P, Sausse A, Funckbrentano JL: Experimental chronic renal-failure in rat by electrocoagulation of renal cortex. Kidney Int 1978;14:82–86.
[PubMed]
5.
Miller PL, Rennke HG, Meyer TW: Glomerular hypertrophy accelerates hypertensive glomerular injury in rats. Am J Physiol 1991;261:F459–F465.
6.
Griffin KA, Picken M, Bidani AK: Method of renal mass reduction is a critical modulator of subsequent hypertension and glomerular injury. J Am Soc Nephrol 1994;4:2023–2031.
[PubMed]
7.
Kumano K, Kogure K, Tanaka T, Sakai T: A new method of inducing experimental chronic-renal-failure by cryosurgery. Kidney Int 1986;30:433–436.
[PubMed]
8.
Brenner BM: Nephron adaptation to renal injury or ablation. Am J Physiol 1985;249:F324–F337.
[PubMed]
9.
Sancho JJ, Duh QY, Oms L, Sitgesserra A, Hammond ME, Arnaud CD, Clark OH: A new experimental model for secondary hyperparathyroidism. Surgery 1989;106:1002–1008.
[PubMed]
10.
Lopezhilker S, Galceran T, Chan YL, Rapp N, Martin KJ, Slatopolsky E: Hypocalcemia may not be essential for the development of secondary hyperparathyroidism in chronic-renal-failure. J Clin Invest 1986;78:1097–1102.
[PubMed]
11.
Peerce BE, Weaver L, Clarke RD: Effect of 2′-phosphophloretin on renal function in chronic renal failure rats. Am J Physiol 2004;287:F48–F56.
You do not currently have access to this content.