... NaOH followed by ultracentrifugation (135,500 g , 1 h , 4°C) to remove any residual aggregates. The soluble Aβ monomers in the supernatant were carefully removed and their concentration determined via the specific absorbance of Aβ at 280 nm (1,730 M - 1 cm - 1 ). Aβ monomer stock solutions were...

... ALS patients. Objective: To investigate the possible pathophysiological linkage between pathologic cytoplasmic inclusions containing TDP-43 and TGFβ/Smad signaling. Methods: We replicated cytoplasmic aggregates of TDP-43 in HEK293T cells by transfecting the cells with a nuclear localization signal...

... apoptotic pathways were elicited by both peptides within time frames correlating with their aggregation properties and formation of oligomeric/protofibrillar assemblies. Our data revealed a primary activation of caspase-8 (typically triggered by death receptors) with secondary engagement of caspase-9...

...Astrid Sydow; Eva-Maria Mandelkow Background: Aggregates of the tau protein are a hallmark of Alzheimer’s and several other neurodegenerative diseases. Various transgenic mouse models have been generated to study the aggregation process. Since wild-type tau is highly soluble and does not aggregate...