Objective: The aim of this study was to investigate the antioxidant activity of extracts of various parts of Acanthopanax trifoliatus obtained by different extraction methods. The total phenolic and flavonoid contents of the extracts were also determined. Materials and Methods: The leaves, stems, stem bark, roots and root bark of A. trifoliatus were extracted separately using decoction, maceration and refluxing methods. The extracts were analysed for free-radical-scavenging activity using the 1,1-diphenyl-2-picryl-hydrazyl scavenging assay and the thiobarbituric-acid-reactive substance method for the inhibition of lipid peroxidation of a rat brain homogenate. Total phenolic and total flavonoid contents of the extracts were measured by UV spectrophotometry. Results: The leaf decoction extracts possessed a significantly stronger antioxidant activity as revealed by both methods. Total phenolic and flavonoid contents of the extracts ranged from 2.16 to 21.79 g% chlorogenic acid equivalent and from 0.37 to 9.61 g% rutin equivalent, respectively. Analysis of the leaf decoction extract, which exhibited the most potent antioxidant activity, by thin-layer chromatography revealed flavonoid and polyphenolic compounds corresponding to rutin and chlorogenic acid. Conclusion: The leaf aqueous extracts showed a high level of antioxidative activity and contained high levels of both phenolic and flavonoid compounds. The magnitude of antioxidant activity corresponded with the level of phenolic and flavonoid compounds.

Keywords:
Acanthopanax trifoliatus, Antioxidant activity, 1,1-Diphenyl-2-picryl-hydrazyl assay, Lipid peroxidation
This content is only available via PDF.
© 2009 S. Karger AG, Basel
2009
Copyright / Drug Dosage / Disclaimer
Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.
You do not currently have access to this content.