...Eugene Y. Wu; Angela K. Hilliker Rifampicin is an effective antibiotic against mycobacterial and other bacterial infections, but resistance readily emerges in laboratory and clinical settings. We screened Escherichia coli for rifampicin resistance and identified numerous mutations to the gene...

...., 2011]) and starvation-stress protocol, rather than use of the tet versus the lac allele, because a comparison of stress-induced reversion of these two alleles in the F indicated that lac was somewhat more mutationally active than tet [Foster, 1997]. That is the opposite of our results with the two...

... construction of multiple, unmarked mutations in the same chromosome, and thus aid in the generation of live vaccine strains or food-safe bacteria. The high-specificity of the Flp- FRT system makes it also applicable for manipulation of whole genomes, including in vivo cloning of large genomic segments...