... of omnipresent gut commensal Escherichia coli by analyzing the relative expression levels of the key genes, which control over twenty important metabolic pathways. Treatment with NAS preparation (aspartame and ace-K) modulates the growth of E. coli as well as inducing the expression of important metabolic genes...

... for highly purified and biologically active hCKMB in the field of diagnostics makes its production valuable. Currently, the production of hCKMB is mainly achieved in methylotrophic yeast, Pichia pastoris , because the production in Escherichia coli is challenging and generally yields an inactive enzyme...

...Eugene Y. Wu; Angela K. Hilliker Rifampicin is an effective antibiotic against mycobacterial and other bacterial infections, but resistance readily emerges in laboratory and clinical settings. We screened Escherichia coli for rifampicin resistance and identified numerous mutations to the gene...

..., and functionally expressed in Escherichia coli . The laccase was highly induced in the E. coli expression system with a maximum intracellular activity of 16 U mg -1 protein. The optimal temperature and pH of the purified laccase were 40°C and 4.6, respectively, when ABTS (2,2'-azino-bis[3-ethylbenzothiazoline-6...

...Fabien Baldacci-Cresp; Anaxi Houbaert; Amana Metuor Dabire; Adeline Mol; Daniel Monteyne; Mondher El Jaziri; Laurence Van Melderen; Marie Baucher Background/Aims: The Escherichia coli MazF is an endoribonuclease that cleaves mRNA at ACA sequences, thereby triggering inhibition of protein synthesis...

... been hindered because ΔlacA strains do not display a clear phenotype to follow. Nevertheless, sugar acetylation and efflux is a general phenomenon involved in the metabolism of other sugars like maltose. Escherichia coli cells grown in the presence of maltose as the unique carbon source export...

...Susy Beatriz Carmona; Fabián Moreno; Francisco Bolívar; Guillermo Gosset; Adelfo Escalante Laboratory and industrial cultures of Escherichia coli employ media containing glucose which is mainly transported and phosphorylated by the phosphotransferase system (PTS). In these strains, 50...

... by the PTS and cAMP. Phosphotransferase system Enzyme II complex Escherichia coli PTS50 symposium NIH Fifty years ago, a novel sugar-phosphorylating system was discovered in Escherichia coli [Kundig et al., 1964]. The unique features of this phosphotransferase system (PTS...

... or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements. 2014 Escherichia coli Long...

...Chun-Yi Lin; Naoki Awano; Hisako Masuda; Jung-Ho Park; Masayori Inouye HipB is a DNA-binding protein in Escherichia coli and negatively regulates its own promoter by binding to the palindromic sequences [<under>TATCC</under>N 8 <under>GGATA</under> (N represents any...

...Hiroshi Nagamitsu; Masayuki Murata; Tomoyuki Kosaka; Junpei Kawaguchi; Hirotada Mori; Mamoru Yamada σ E -dependent cell lysis has been proposed to eliminate damaged cells in the stationary phase in Escherichia coli . In order to explore the relationship of this process to long-term stationary phase...

...) to the periplasmic and, eventually, extracellular space of Escherichia coli . Eight mutant SPs were constructed using site-directed mutagenesis to improve the secretion of recombinant CGTase. M5 is a mutated SP in which replacement of an isoleucine residue in the h-region to glycine created a helix-breaking or G...

...Akira Iwamoto; Atsushi Osawa; Makiko Kawai; Hirofumi Honda; Saori Yoshida; Nobuhisa Furuya; Jun-ichi Kato The Escherichia coli yqgF gene is highly conserved across a broad spectrum of bacterial genomes. The gene was first identified as being essential for cell growth during screening for targets...

... by Escherichia coli as carrier chassis, a modified transporter was designed with the ability to secrete biologically active IL-10. De novo DNA synthesis comprised a 561-bp fragment encoding the signal sequence of the E. coli outer membrane protein F fused in frame to an E. coli codon-optimized mature human IL-10...

..., transferring electrons to ubiquinone (UQ) in the membrane. Based on the sequence homology of the C-terminal catalytic domain (151–796 amino acid residues) we have modeled the 3D structure of Escherichia coli GDH. The geometrical parameters of the homology model structure, validated using the Ramachandran plot...

...Tuan A. Nguyen; Samantha S. Lieu; Geoffrey Chang A cell-free expression system using an Escherichia coli extract was adapted for large-scale expression and purification of mammalian membrane proteins. The system was tested with a set of human membrane proteins of different sizes, numbers...

... Porin Escherichia coli Salmonella enterica Research Article J Mol Microbiol Biotechnol 2010;18:24 36 DOI: 10.1159/000274309 The Complexities of Porin Genetic Regulation Miguel Ángel De la Cruz Edmundo Calva Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, México...

...Bożena Nejman; Joanna M. Łoś; Marcin Łoś; Grzegorz Węgrzyn; Alicja Węgrzyn Background: Genes encoding Shiga toxins in pathogenic Escherichia coli strains (Shiga toxin-producing E. coli , STEC) are located on lambdoid prophages. However, studies on the replication of these phages were not reported...

...Rashed Noor; Masayuki Murata; Mamoru Yamada σ E , encoded by rpoE , causes lysis of viable but nonculturable (VBNC) cells at the early stationary phase in Escherichia coli . However, the factors that trigger this lysis phenomenon remain unidentified. In this study, we found that the defective...

...Gerardo Huerta-Beristain; José Utrilla; Georgina Hernández-Chávez; Francisco Bolívar; Guillermo Gosset; Alfredo Martinez Modification of ethanol productivity and yield, using mineral medium supplemented with glucose or xylose as carbon sources, was studied in ethanologenic Escherichia coli KO11...