Abstract
The polysaccharide capsule of Streptococcus pneumoniae is one of the most important virulence factors responsible for human infections and in mouse infection models as well. Larvae of Manduca sexta were used as an alternative animal model in order to test the impact of the pneumococcal capsule on virulence in the insect host. The unencapsulated S. pneumoniae strain R6 was able to cause disease and induce killing in the larvae, and similar results were obtained with related commensal species. However, using the same dose of S. pneumoniae, encapsulated strains including the type 2 D39 strain, the progenitor of R6, and genetically unrelated S. pneumoniae strains of serotype 2, 4, 6B, 23F and 19A, all had increased virulence potential compared to the R6 strain. Between 20 and 70% of the larvae were affected after 96 h compared to 12% observed with R6. Two type 6B S. pneumoniae strains were more virulent compared to the other strains. S. pneumoniae R6 transformants producing the type 6B capsule showed a similar elevated disease potential, confirming the contribution of the pneumococcal polysaccharide capsule to virulence in M. sexta.
Journal Section:
Research Article
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