... and a portion of this was purified by column chromatography using DEAE-Sephadex A-50. Both the purified hemoglobin (10 –5 M) and crude erythrolysate (the latter diluted appropriately in Krebs solution to contain 10 – 5 M hemoglobin) reduced lymphatic fluid pumping approximately 70% over a period of 2 h...

... of removing endothelial cells. In the perfused mesenteric arterial vasculature of the rat, vasodilation by ACh was markedly, though not completely, inhibited by hemoglobin (10 µ M ), an agent which can inactivate endothelium-derived relaxing factor (EDRF). These results suggest that a major component...

... function by gossypol or the presence of albumin. It is therefore unlikely that the flow-induced constriction results from a ‘wash away’ effect of endothelium-derived relaxing factor (EDRF). To test whether EDRF could still play a role after gossypol, we used hemoglobin (n = 5) to bind EDRF. Flow-dependent...

...Jaroslav Pelisek; Sorin Armeanu; Sigrid Nikol The reporter gene for β-galactosidase is frequently used to determine the efficiency of gene transfer in arteries. However, blood is often present in arterial explants and may compromise the results by the presence of hemoglobin. The light absorption...