... rats were exposed to cigarette smoke for 30 min/day, 5 days a week. Diabetes was induced by the intravenous injection of streptozotocin. After 16 weeks, the abdominal aorta was collected for biomechanical, histological, and matrix metalloproteinase 2 (MMP-2) activity analyses. Results: The valid...

... adaptive changes in aortic biomechanics that correlate with the Tet family gene expression. Methods: Abdominal aortas from pregnant and nonpregnant mice were dissected and cannulated. Intraluminal pressure was adjusted using a pressure-servo system while using a video dimension analyzer to measure...

... effectiveness and matrix structure preservation were histologically examined. Mechanical tests were performed. Decellularized porcine grafts were interpositioned in a porcine aorta. After a 6-month period, implanted samples were removed and evaluated using light and electron microscopy. Results: Histological...

... such as in atherosclerosis. Thus, laboratories around the world measure vascular contractile function in vitro, wherein an isolated blood vessel, e.g. aorta, is contracted with an agonist, e.g. phenylephrine (PE), endothelin-1, U46,619 – a thromboxane A 2 analog, etc… and data reported [ 3-5 ]. The widespread use...

... were sacrificed, and the aortas were isolated and then digested to analyze aortic cell uptake of AngioSPARK by flow cytometry. Results: CD11b-expressing aortic macrophages from mice injected with AngioSPARK showed high levels of fluid-phase pinocytosis compared to aortic cells not expressing CD11b...

...-induced relaxation in the mouse aorta, (2) the effect of atherosclerosis on relaxation to peroxynitrite and other vasodilators, and (3) the effect of atherosclerosis on the expression and function of the IP3 receptor. Aortic function was studied using wire myography, and atherosclerosis was induced by fat...

... or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements. Valsartan Chronic kidney disease Aorta Vascular remodeling Endothelium Nitric oxide...

... hypothesized that LXA 4 promotes contractile responses and contributes to endothelial dysfunction. Methods: We used aorta from Wistar rats to assess vascular function. Reactive oxygen species (ROS) production and contractile and regulatory proteins were investigated. Results: LXA 4 induced concentration...

...Eduardo Oliver; Nicla Flacco; Cristina Arce; M. Dolores Ivorra; M. Pilar D'Ocon; M. Antonia Noguera This work compares the expression of adrenoceptors (ARs) and G-protein-coupled receptor kinase (GRK) 2 (RT-PCR and immunoblotting) and functional responses in conductance (aorta) and resistance...

...Luciano Figueiredo Borges; João Paulo Forato Blini; Ricardo Ribeiro Dias; Paulo Sampaio Gutierrez In ascending aorta aneurysms (AscAA) the whole vessel wall dilates, while in aortic dissections (AD) the wall cleaves into two sheets. Both may present fine elastic fragmentation and a decrease...

...Anneloes Martinsen; Xavier Yerna; Géraldine Rath; Elvira Leon Gomez; Chantal Dessy; Nicole Morel In addition to its role in the regulation of artery contraction, Rho kinase (ROCK) was reported to be involved in the cytosolic calcium response to vasoconstrictor agonists in rat aorta and superior...

... status and NO bioavailability is unclear. Thus, we aimed to evaluate vascular reactivity in mice lacking functional β 2 -AR (β 2 KO), focusing on the role of NO and superoxide anion. Methods and Results: Isolated thoracic aortas from β 2 KO and wild-type mice (WT) were studied. β 2 KO aortas exhibited...

... ADAMs in AAA. Results: ADAMs tested in our study were expressed in both AAA and control aorta without any significant differences between the groups. In contrast, expression of TIMP-1 was significantly reduced in AAA compared to control vessels. Smooth muscle cells (SMCs), neovessels and macrophages...

... or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements. Aorta Elastin Ageing Vascular ageing Vascular imaging Cardiovascular disease...

... in the dark on a rotating holder at 4°C. Aorta Aortic adventitial fibroblasts Macrophages Angiotensin II Interleukin-6 MCP-1 CCR2 National Institutes of Health (NIH) 10.13039/100000002 Little is known about the contribution of adventitial fibroblasts to vascular wall...

... structure, and abdominal aortic wall dynamics in vivo. Methods: Elastin (ELN) transcript levels were quantified by qRT-PCR and blood pressure was measured with a tail cuff system. M-mode ultrasound was used to track pulsatile abdominal aortic wall motion. Aortas were sectioned and stained to determine...

... significantly more PDGF produced by the distal compared to proximal aorta at 77 ± 10 versus 14 ± 6 pg/cm 2 /72 h (p < 0.05). Endothelial cells (EC) and smooth muscle cells (SMC), isolated from analogous aortic sites, were grown in tissue culture and the conditioned medium was assayed for PDGF. EC in vitro...

... or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements. Diabetes Vascular hypertrophy Angiotensin II Mesentery Aorta...

... and the hypervariable region of the rat biglycan core protein. Western blot analysis of proteoglycans isolated from bovine and rat aortas by ion exchange chromatography and treated with chondroitin ABC lyase, with antibody against propeptide, demonstrated core proteins with molecular weights ranging from 43,000...

... ET-1 were found with progression of hypertension in aorta and the mesenteric arterial bed only in unilaterally nephrectomized DOCA-salt-treated rats (DOCA-salt-hypertensive rats). Vascular expression of ET-1 was not enhanced in other DOCA- or salt-treated rats, even when blood pressure rose to a mean...