... of muscarinic receptors. During similar contractions to phenylephrine, the endothelium-dependent agonist acetylcholine-induced relaxations were significantly enhanced in segmental renal arteries of lean but not obese CA-AMPK mice compared to corresponding preparations from WT littermates (Fig.  3...

... vascular dysfunction. Methods and Results: This study characterized force maintenance and the sensitivity to acetylcholine (ACh)-mediated smooth muscle (SM) relaxation of tertiary (3rd) mesenteric arteries from Sprague-Dawley rats following 30 min of myocardial ischemia. Both the phosphorylation...

...A. Rousseau; E. Tesselaar; J. Henricson; F. Sjöberg Hyperoxia causes vasoconstriction in most tissues, by mechanisms that are not fully understood. We investigated microvascular effects of breathing 100% oxygen in healthy volunteers, using iontophoresis to deliver acetylcholine (ACh) and sodium...

...). Methods: Insulin sensitivity and endothelial function was studied with venous occlusion plethysmography and intra-arterial infusions of adenosine and acetylcholine, before and during a hyperinsulinemic isoglycemic clamp. Results: Forearm blood flow response to systemic hyperinsulinemia was diminished...

... model. Results: The model accurately describes the blood flow responses to acetylcholine and sodium nitroprusside during a single iontophoretic current pulse. There is a significant clearance out of the microvascular bed during iontophoresis which depends on the type of drug administered. Conclusion...

... by adding acetylcholine or bradykinin in the bath. On the other hand, the effect of a stepwise increase in intraluminal flow (50–450 µl/min) of physiological salt solution was observed. Agonist-induced dilations were significantly smaller in arteries of SHRs compared to those of WKY rats. Starting flow...

...Mercedes Ferrer; Araceli Encabo; María V. Conde; Jesús Marín; Gloria Balfagón The possible endothelial factors involved in endothelium-dependent relaxations induced by acetylcholine (ACh) in aorta, mesenteric and femoral arteries of rabbit were analyzed. In thoracic aorta precontracted...

... of the gap junction inhibitors octanol (10 -4 mol/l) and heptanol (10 -3 mol/l) were examined on acetylcholine-, the NO-donor S-nitroso-N-acetyl-penicillamine (SNAP)-, and guanosine-3’, 5’-cyclic monophosphate (cGMP)-induced relaxation. In addition, we tested varying concentrations of the gap junction...

...Angel Luis García-Villalón; Nuria Fernández; Luis Monge; José Luis García; Bernardino Gómez; Godofredo Diéguez The main objective of this work was to study the role of potassium channels in the cholinergic relaxation of cutaneous arteries during cooling. Acetylcholine (10 –8 –10 –4 M ) produced...

...M.A. Rodríguez-Martínez; J. Martínez-Orgado; M. Salaices; J. Marín The effect of malondialdehyde (MDA), a lipid peroxidation marker, on the relaxations evoked by acetylcholine (ACh) was analyzed in tail arteries of Sprague-Dawley rats, which have MDA plasma levels of 0.43 ± 0.10 µ M . MDA (0.5–30 µ...

... superfusion with ADP, acetylcholine and nitroglycerin before and during topical application of inhibitors of nitric oxide synthesis (N G -monomethyl- L -arginine; L-NMMA and N w -nitro-L-arginine; L-NNA). Prior to suffusion with L-NMMA and L-NNA, ADP (10 and 100 µ M ) , acetylcholine (0.1 and 1.0 µ M...

... added. The change in the outer diameter of the vessels [preconstricted with norepinephrine (NE) to 70%] in response to acetylcholine as an indicator of the endothelial function, was repeatedly measured over 5 h after the equilibration. The difference between the acetylcholine responses of the two vessel...

...Hui Xie; Christopher R. Triggle The effects of acetylcholine (ACh) and A23187 on ring preparations from the human umbilical artery (HUA) were investigated and compared with the rat aorta (RA). The results from the HUA demonstrate that: (1) At both high (pO 2 > 600 mm Hg) and low O 2 tension pO 2...

... a neo-intima similar to that of human early atherosclerosis, and carotid blood flow was measured periodically over 8 days in 8 conscious rabbits. The vasodilator responses to intravenous infusions of the endothelium-dependent vasodilator, acetylcholine, and glyceryl trinitrate were measured in each...

... isolated from rabbits subjected to ischemia and reperfusion of one lung. Percent relaxation values of sham-operated (i.e. nonischemic) pulmonary arteries to endothelium-dependent vasodilators acetylcholine (ACh) and A23187 were 72 ± 4 and 65 ± 4%, respectively, while relaxation to the endothelium...

...Eric O. Feigl; Donna M. Van Winkle; Jody K. Miyashiro Intracoronary infusion of acetylcholine produces a prompt increase in coronary blood flow in dogs, but a paradoxical decrease has been reported in baboons and cattle. The action of acetylcholine was reexamined in anesthetized dogs, baboons...

... response of pial arterioles to the topical application of acetylcholine (ACh; 10 –7 M ), an endothelial-dependent vasodilator, was measured using a closed cranial window technique. Although ACh increased pial arteriolar caliber by 17 % under resting conditions, the same dose elicited a vasoconstrictor...

...–12 months. The contractile response to noradrenaline (NA) decreased with increasing age, but NA [EC 50 ] did not vary significantly. Acetylcholine (Ach)-induced relaxation of aortic rings precontracted with NA [EC 50 ] did not change significantly with increasing age. The relaxation induced by ATP...

...Jianming Xie; Ye Wang; Stanley S. Greenberg SKF-525A (proadifen) inhibits endothelium-dependent relaxations induced by acetylcholine, arachidonic acid and the calcium ionophore A23187. This suggests that SKF-525A is an inhibitor of endothelium-derived relaxing factor (EDRF) and that EDRF may...

... lengths. The decrease in mean cell length of dispersed cells exposed to serotonin, acetylcholine, and ergonovine maleate was 21.0, 23.0 and 25.5%, respectively (p < 0.05). No significant decrease in cell length was obtained with norepinephrine. The ultrastructure of the relaxed cells seemed...