Background/Aims: The influence of TAS2R38 haplotype on the relationship between the perceived intensity of propylthiouracil (PROP) and the basic tastes of salt, sweet, sour, and bitter (quinine) was evaluated in the Beaver Dam Offspring Study. Methods: Genotyping was performed on 1,670 participants aged ≥45 years (mean age = 54.4; range = 45-84), and suprathreshold taste intensity was measured using filter paper disks and a general labeled magnitude scale (0-100). Results: Among those with taste intensity data and the PAV or AVI haplotype (n = 1,258), the mean perceived intensity of PROP was 37.3 (SD = 30.0), but it varied significantly (p < 0.0001) by diplotype (PAV/PAV = 60.1; PAV/AVI = 46.5; AVI/AVI = 14.4). PROP intensity was correlated with the basic taste intensities (salt: r = 0.22; sweet: r = 0.25; sour: r = 0.21; quinine bitterness: r = 0.38; p < 0.001 for all tastes); however, a significant effect modification of the PROP-taste intensity relationships by TAS2R38 diplotype was observed. There was a stronger association between PROP and each of the basic tastes in the PAV/PAV diplotype group than in the other groups. Conclusions: Directly measuring the perceived intensity of the 4 tastes, rather than using PROP intensity as an indicator of taste responsiveness, is recommended for studies of taste perception.

Keywords:
Taste, Propylthiouracil, Population association study
1.
Glanz K, Basil M, Maibach E, Goldberg J, Snyder D: Why Americans eat what they do: taste, nutrition, cost, convenience, and weight control concerns as influences on food consumption. J Am Diet Assoc 1998;98:1118-1126.
2.
Dressler H, Smith C: Food choice, eating behavior, and food liking differs between lean/normal and overweight/obese, low-income women. Appetite 2013;65:145-152.
3.
Peyrot des Gachons CP, Beauchamp GK, Breslin PA: The genetics of bitterness and pungency detection and its impact on phytonutrient evaluation. Ann NY Acad Sci 2009;1170:140-144.
4.
Blakeslee AF: Genetics of sensory thresholds: taste for phenylthiocarbamide. Proc Natl Acad Sci USA 1932;18:120-130.
5.
Kim UK, Jorgenson E, Coon H, Leppert M, Risch N, Drayna D: Positional cloning of the human quantitative trait locus underlying taste sensitivity to phenylthiocarbamide. Science 2003;299:1221-1225.
6.
Duffy VB, Davidson AC, Kidd JR, Kidd KK, Speed WC, Pakstis AJ, Reed DR, Snyder DJ, Bartoshuk LM: Bitter receptor gene (TAS2R38), 6-n-propylthiouracil (PROP) bitterness and alcohol intake. Alcohol Clin Exp Res 2004;28:1629-1637.
7.
Calo C, Padiglia A, Zonza A, Corrias L, Contu P, Tepper BJ, Barbarossa IT: Polymorphisms in TAS2R38 and the taste bud trophic factor, gustin gene co-operate in modulating PROP taste phenotype. Physiol Behav 2011;104:1065-1071.
8.
Reed DR, Nanthakumar E, North M, Bell C, Bartoshuk LM, Price RA: Localization of a gene for bitter-taste perception to human chromosome 5p15. Am J Hum Genet 1999;64:1478-1480.
9.
Bufe B, Breslin PA, Kuhn C, Reed DR, Tharp CD, Slack JP, Kim UK, Drayna D, Meyerhof W: The molecular basis of individual differences in phenylthiocarbamide and propylthiouracil bitterness perception. Curr Biol 2005;15:322-327.
10.
Bartoshuk LM, Duffy VB, Miller IJ: PTC/PROP tasting: anatomy, psychophysics, and sex effects. Physiol Behav 1994;56:1165-1171.
11.
Bartoshuk LM, Duffy VB, Lucchina LA, Prutkin J, Fast K: PROP (6-n-propylthiouracil) supertasters and the saltiness of NaCl. Ann NY Acad Sci 1998;855:793-796.
12.
Bartoshuk LM: Comparing sensory experiences across individuals: recent psychophysical advances illuminate genetic variation in taste perception. Chem Senses 2000;25:447-460.
13.
Delwiche JF, Buletic Z, Breslin PAS: Relationship of papillae number to bitter intensity of quinine and PROP within and between individuals. Physiol Behav 2001;74:329-337.
14.
Bajec MR, Pickering GJ: Thermal taste, PROP responsiveness, and perception of oral sensations. Physiol Behav 2008;95:581-590.
15.
Hayes JE, Bartoshuk LM, Kidd JK, Duffy VB: Supertasting and PROP bitterness depends on more than the TAS2R38 gene. Chem Senses 2008;33:255-265.
16.
Lucchina LA, Curtis OF 5th, Putnam P, Drewnowski A, Prutkin JM, Bartoshuk LM: Psychophysical measurement of 6-n-propylthiouracil (PROP) taste perception. Ann NY Acad Sci 1998;855:816-819.
17.
Hayes JE, Sullivan BS, Duffy VB: Explaining variability in sodium intake through oral sensory phenotype, salt sensation and liking. Physiol Behav 2010;100:369-380.
18.
Tepper BJ, Nurse RJ: PROP taster status is related to fat perception and preference. Ann NY Acad Sci 1998;855:802-804.
19.
Hayes JE, Duffy VB: Revisiting sugar-fat mixtures: sweetness and creaminess vary with phenotypic markers of oral sensation. Chem Senses 2007;32:225-236.
20.
Yackinous C, Guinard J-X: Relation between PROP taster status and fat perception, touch, and olfaction. Physiol Behav 2001;72:427-437.
21.
Drewnowski A, Henderson SA, Cockroft JE: Genetic sensitivity to 6-n-propylthiouracil has no influence on dietary patterns, body mass indexes, or plasma lipid profiles of women. J Am Diet Assoc 2007;107:1340-1348.
22.
Lim J, Urban L, Green BG: Measures of individual differences in taste and creaminess perception. Chem Senses 2008;33:493-501.
23.
Essick GK, Chopra A, Guest S, McGlone F: Lingual tactile acuity, taste perception, and the density and diameter of fungiform papillae in female subjects. Physiol Behav 2003;80:289-302.
24.
Duffy VB, Hayes JE, Davidson AC, Kidd JR, Kidd KK, Bartoshuk LM: Vegetable intake in college-aged adults is explained by oral sensory phenotypes and TAS2R38 genotype. Chemosens Percept 2010;3:137-148.
25.
Fischer ME, Cruickshanks KJ, Schubert CR, Pinto A, Klein R, Pankratz N, Pankow JS, Huang GH: Factors related to fungiform papillae density: the Beaver Dam Offspring Study. Chem Senses 2013;38:669-677.
26.
Garneu NL, Nuessle TM, Sloan MM, Santorico SA, Coughlin BC, Hayes JE: Crowdsourcing taste research: genetic and phenotypic predictors of bitter taste perception as a model. Front Integr Neurosci 2014;8:33.
27.
Cruickshanks KJ, Wiley TL, Tweed TS, Klein BEK, Klein R, Mares-Perlman JA, Nondahl DM: Prevalence of hearing loss in older adults in Beaver Dam, Wisconsin. The Epidemiology of Hearing Loss Study. Am J Epidemiol 1998;148:879-886.
28.
Cruickshanks KJ, Schubert CR, Snyder DJ, Bartoshuk LM, Huang G-H, Klein BEK, Klein R, Nieto FJ, Pankow JS, Tweed TS, Krantz EM, Moy GS: Measuring taste impairment in epidemiologic studies. The Beaver Dam Offspring Study. Ann NY Acad Sci 2009;1170:543-552.
29.
Zhan W, Cruickshanks KJ, Klein BEK, Klein R, Huang GH, Pankow JS, Gangnon RE, Tweed TS: Generational differences in the prevalence of hearing impairment in older adults. Am J Epidemiol 2010;171:260-266.
30.
Keating BJ, Tischfield S, Murray SS, Bhangale T, Price TS, Glessner JT, et al: Concept, design and implementation of a cardiovascular gene-centric 50 K SNP array for large-scale genomic association studies. PLoS One 2008;3:e3583.
31.
Purcell S, Neale B, Todd-Brown K, Thomas L, Ferreira MAR, Bender D, Maller J, Sklar P, de Bakker PIW, Daly MJ, Sham PC: PLINK: a tool set for whole-genome association and population-based linkage analyses. Am J Hum Genet 2007;81:559-575.
32.
Price AL, Patterson NJ, Plenge RM, Weinblatt ME, Shadick NA, Reich D: Principal components analysis corrects for stratification in genome-wide association studies. Nat Genet 2006;38:904-909.
33.
Patterson N, Price AL, Reich D: Population structure and eigenanalysis. PLoS Genet 2006;2:e190.
34.
Bartoshuk LM, Duffy VB, Green BG, Hoffman HJ, Ko C-W, Lucchina LA, Marks LE, Snyder DJ, Weiffenbach JM: Valid across-group comparisons with labeled scales: the gLMS versus magnitude matching. Physiol Behav 2004;82:109-114.
35.
Drewnowski A, Kristal A, Cohen J: Genetic taste responses to 6-n-propylthiouracil among adults: a screening tool for epidemiological studies. Chem Senses 2001;26:483-489.
36.
Fischer ME, Cruickshanks KJ, Schubert CR, Pinto A, Klein BE, Klein R, Nieto FJ, Pankow JS, Huang GH, Snyder DJ: Taste intensity in the Beaver Dam Offspring Study. Laryngoscope 2013;123:1399-1404.
37.
Prutkin J, Duffy VB, Etter L, Fast K, Gardner E, Lucchina LA, Snyder DJ, Tie K, Weiffenbach J, Bartoshuk LM: Genetic variation and inferences about perceived taste intensity in mice and men. Physiol Behav 2000;69:161-173.
38.
Ohla K, Lundström JN: Sex differences in chemosensation: sensory or emotional? Front Hum Neurosci 2013;7:607.
39.
Duffy VB, Peterson JM, Bartoshuk LM: Associations between taste genetics, oral sensation and alcohol intake. Physiol Behav 2004;82:435-445.
40.
Drewnowski A, Henderson SA, Shore AB: Genetic sensitivity to 6-n-propylthiouracil (PROP) and hedonic responses to bitter and sweet tastes. Chem Senses 1997;22:27-37.
41.
Kaminski LC, Henderson SA, Drewnowski A: Young women's food preferences and taste responsiveness to 6-n-propylthiouracil (PROP). Physiol Behav 2000;68:691-697.
42.
Ly A, Drewnowski A: PROP (6-n-Propylthiouracil) tasting and sensory responses to caffeine, sucrose, neohesperidin dihydrochalcone and chocolate. Chem Senses 2001;26:41-47.
43.
Marks LE, Borg G, Westerlund J: Differences in taste perception assessed by magnitude matching and by category-ratio scaling. Chem Senses 1992;17:493-506.
44.
Hayes JE, Keast RS: Two decades of supertasting: where do we stand? Physiol Behav 2011;104:1072-1074.
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