An in vitro bioassay method using Leydig cells from mouse testes has been validated for quantitating luteinizing hormone (LH) in the plasma and cerebrospinal fluid(CSF) of normally menstruating and ovariectomized rhesus monkeys. In all six normally menstruating monkeys studied, the mid-cycle peak of plasma LH coincided with the estradiol peak in blood samples taken at 24-hour intervals. Blood samples collected at 4-hour intervals during the periovulatory period showed multiple peaks of both bioactive LH and estradiol, and the major estradiol peak preceded the major bioactive LH peak by about 16 h. The plasma progesterone levels were about 1 ng/ml at the time of LH peak. Bioactive LH was detected also in the CSF samples of both intact and ovariectomized monkeys. The levels of LH in CSF were not elevated in the gonadectomized monkeys although their plasma LH levels were markedly increased.

Keywords:
Bioactive LH, Menstrual cycle, Rhesus monkey, Cerebrospinal fluid
This content is only available via PDF.
1980
Copyright / Drug Dosage / Disclaimer
Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.
You do not currently have access to this content.