...Kunikazu Tanji; Keiichi Saekia; Yasunobu Matsumoto; Makio Takeda; Kensuke Hirasawa; Kunio Doi; Yoshitsugu Matsumoto; Takashi Onodera An amyloid-like isoform of a 33- to 34-kD glycoprotein, termed as the scrapie prion protein (PrP sc ), plays a critical role in transmissible spongiform...

... spongiform encephalopathy specific amyloid protein Scrapie Nucleic acid Intervirology Original Paper Intervirology 1997;40:238-246 Received: September 18, 1997 Accepted after revision: February 4, 1998 Heino Diringer3 Michael Beekes3 Muhsin Ozela Dietrich Simon3 Ingo Quecka Franco Cardoneb Maurizio...

...Harash K. Narang; Neil S. Millar; David M. Asher; Carleton Gajdusek We observed a marked increase in multimeric mitochondrial DNA (mtDNA) in brains of scrapie-infected hamsters compared with those of uninfected hamsters. Homogenized brain tissue was subjected to subcellular fractionation to isolate...

...Harash K. Narang Examination of thin sections from the cerebral cortex of scrapie-infected hamster brains revealed characteristic circular 26–30 nm diameter tubulofilamentous particles, identical to those previously described in both experimentaly induced scrapie in mice, hamsters and natural...

...Charles Weissmann; Hansruedi Büeler; Marek Fischer; Michel Aguet The transmissible agent that causes spongiform encephalopathies such as scrapie, the prion, is believed to be devoid of nucleic acid and identical with PrP Sc , a modified form of PrP c . PrP c is a normal host protein encoded...

...Harash K. Narang Abnormal tubulofilamentous particles were identified by electron microscopy using a simple touch negative staining technique from brains of mice infected with four strains of the scrapie agent. Treatment by three proteolytic enzymes and subsequent treatment with DNase and mung bean...

... of tubulofilamentous particles. The unmasked fibrils were identified as scrapie-associated fibrils by immunogold labelling. Both tubulofilamentous particles and scrapie-associated fibrils are not artefacts of protease treatment of protein formed in vitro during purification. Treatment with protease and nucleases...

... similar to those observed in brains from hamsters infected with scrapie. These structures are consistently present in naturally occurring and experimentally induced spongiform encephalopathies, irrespective of the host species or virus strain. Their role in pathogenesis is undetermined. 22 08 1987...

...Richard I. Carp; Sharon M. Callahan Injection of mice with thioglycolate 5 days prior to intraperitoneal injection of scrapie brain homogenate led to a statistically significant increase in scrapie incubation periods. This was seen with two different scrapie strains (ME7 and 139A), in different...

...Douglas W. Ehresmann; Nick Hogan Coinfected mice were examined for a possible interaction between the scrapie agent and an adenovirus. A low titer (10 2 TCD 50 ) of mouse adenovirus (MAdV) caused a significant acceleration of clinical signs of scrapie in mice infected 128 days previously...

..., but decreased after intracerebral injection. In contrast, when nucleic acids from scrapie-infected brains were entrapped in liposomes and injected into mice by one of four routes, no cases of scrapie were observed. This is the first report of the enhancement of nucleic acid infectivity by liposomes in vivo...

...Richard I. Carp; Sharon M. Callahan Mouse peritoneal macrophages were exposed to scrapie (ME7 strain) brain homogenate in vitro for 2 h at 37 c . The samples were assayed for infectivity by analysis of scrapie incubation periods and their values compared to those obtained after extended (1–28 days...

...Richard I. Carp; Sharon M. Callahan Scrapie brain homogenate was mixed with mouse peritoneal macrophages in vitro. After 2 h of incubation at 37°, a portion of the scrapie infectivity was associated with macrophages. In contrast, very little infectivity was associated with kidney cells that had...

...John Hotchin; Edward Sikora; Fred Baker Examination of newborn mice, inoculated intraperitoneally with high doses of scrapie virus, revealed that the virus could not be reisolated from their tissues after about 1 week following inoculation, until almost 1 year later. The inoculum was rapidly...

...Hyun Ju Cho Scrapie infectivity in the Hirt supernatant of a 20% scrapie-infected mouse brain filtrate was almost totally inactivated by pronase treatment. It was not affected by RNase A treatment and was only slightly reduced by DNase I treatment. These results indicate that a protein component...

...Robert A. Somerville; Geoffrey C. Millson; Richard H. Kimberlin Exposure of fractions obtained from scrapie-infected mouse brain to Triton X-100 resulted in no change in its infectivity except after sedimentation. Infectivity was reduced after exposure to 2-mercaptoethanol but only in the presence...

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