..., and according the size into groups of small and large lesions. Polymerase chain reaction was used to detect HCMV and EBV. The amplification was performed in a DNA Thermal Cycler (Hybaid). Results: Symptomatic lesions were 7.68 times more likely to be infected with HCMV than asymptomatic lesions (p < 0.001...

..., instructions or products referred to in the content or advertisements. Chronic hepatitis Polymerase chain reaction HCV genotyping Infected carriers Hepatocellular carcinoma Hepatitis C virus (HCV) is one of the leading causes of end-stage liver disease with high progression to cirrhosis...

... polyomavirus (MCPyV). MCPyV has been detected in human tissue samples. However, detailed distribution of MCPyV in non-neoplastic Japanese human tissues remains unclear. To address this, we used single or real-time quantitative polymerase chain reaction (PCR) for 41 autopsy cases. PCR revealed MCPyV-DNA in non...

... used to diagnose HCMV shedding in urines, such as virus culture followed by indirect immunofluorescence and pp65 gene-specific polymerase chain reaction. Although less sensitive than the polymerase chain reaction and cross-reactive with other herpesvirus DNA polymerases, the activity measured...

... Hepatitis C Hemodialysis Polymerase chain reaction Seroconversion Summary Among patients undergoing maintenance hemodialysis, a high prevalence of hepatitis C virus (HCV) infection can be observed. In a prospective study, sera of 273 patients were examined for the presence of HCV infection by serologi­ cal...

...-specific viral glycoproteins, are useful for serological typing, the polymerase chain reaction technique has proved highly effective in giving early, precise and specific HSE diagnosis, thus providing a helpful tool for the identification and treatment of this disease. 30 7 2008 © 1996 S...

... and histology, immunocytochemistry, electron microscopy, serology and methods for the detection of HPV-specific DNA. Among the latter are polymerase chain reaction (PCR), Southern blot, dot blot, filter in situ hybridization, in situ hybridization and direct hybridization assays as Hybrid Capture®...

... and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements. Hepatitis B virus Liver disease Viral mutants Quasispecies DNA sequencing Polymerase chain reaction Vaccine escape...

... by polymerase chain reaction. This method may prove useful for monitoring HCMV encephalitis and neuropathy. Virus Detection, Viral E co lo gy and D isease Intervirology 1993;35:60-72 G. Jahn B. Plachter Institut für Klinische und Molekulare Virologie der Universität Erlangen-Nürnberg, Erlangen, BRD Diagnostics...

...). Some IL and MN strains, but not the NY strain, could be detected by ELISA with a monoclonal antibody raised against BYDV-RPV-NY. The region of the viral genome representing the coat protein gene was amplified by polymerase chain reaction, cloned and sequenced. The nucleotide sequences of the BYDV-RMV...

...M.A. Rezaian; L.R. Krake; D.A. Golino Pairs of viroid-specifíc oligonucleotide primers were selected and used in separate reverse transcription reactions coupled with the polymerase chain reaction to obtain DNA products of predetermined sizes characteristic of each viroid. The reaction conditions...

.... However, a knowledge of the sequence of these clones has been used to synthesize oligonucleotides that have been used for polymerase chain reaction amplification of specific sequences from both purified dsRNA and from infected plants and used as a previously unavailable highly sensitive diagnostic probe...

...Hideaki Kikuta; Masonori Nakanishi; Nobuyoshi Ishikawa; Mutsuko Konno; Shuzo Matsumoto We used a selective DNA amplification technique to detect Epstein-Barr virus (EBV) DNA in peripheral blood mononuclear cells from patients with Kawasaki disease (KD). By means of the polymerase chain reaction EBV...

...Edouard M. Cantin; Winand Lange; Harry Openshaw We have amplified herpes simplex virus type 1 (HSV-1) DNA sequences from individual latently infected mouse trigeminal ganglia by polymerase chain reaction (PCR) assays. This report presents two useful modifications in the PCR technique. The first...

... by quantitative fluorescence polymerase chain reaction (PCR). A fragment of the gB gene was amplified by nested PCR. CMV gB genotyping was carried out by restriction fragment length polymorphism, and 24 samples of the amplified DNA fragments were verified by DNA sequencing. Results: The levels of CMV DNA...

... examination must remain an essential part of the routine screening in hemodialysis patients. Hepatitis C virus Hemodialysis Seroconversion Polymerase chain reaction © 2005 S. Karger AG, Basel 2005 Copyright / Drug Dosage / Disclaimer Copyright: All rights reserved. No part of this publication...

... or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements. Blood products Chimpanzee Hepatitis C virus Polymerase chain reaction...

... in the same reading frame as ORF1. A stretch of 46–96 nucleotides was identified, point mutations and deletions in which were specific for the four genotypes (I–IV). A polymerase chain reaction method was developed with 9 nested universal primers, deduced from conserved regions in the 5′-terminal sequences...

... antibiotic treatment on the other. Diagnostic procedures for NV are based on the detection of virus in stool samples by (immune) transmission electron microscopy (TEM), antigen ELISA, or polymerase chain reaction (PCR). In our study, a total of 244 stool samples obtained from 227 patients between March...

... Immunofluorescence assay, indirect Polymerase chain reaction RT-PCR Original Paper Intervirology 2003;46:227 231 DOI: 10.1159/000072432 Received: September 12, 2002 Accepted after revision: May 22, 2003 Improved Detection of Dengue-1 Virus from IgM-Positive Serum Samples Using C6/36 Cell Cultures in Association...