... information about the distribution and diversity of herpesviruses in wild rodent and shrew species in central Africa, animals in Cameroon and the Democratic Republic of the Congo were sampled and tested by PCR for the presence of herpesvirus DNA. Methods: A broad range PCRs targeting either the Polymerase...

..., Upper Egypt. Methods: HBsAg and HBeAg were examined by ELISA while HBV-DNA was examined by PCR. HBV genotyping was conducted by restriction fragment length polymorphism. Results: HBsAg was detected in 237 donors (1.98%). The HBV-DNA of 50 donors with the highest HBsAg OD was examined for the HBV...

... A, Faure C, Marianneau P, Buckland R, Lam S, Wild TF, Deubel V: Specific detection of Nipah virus using real-time RT-PCR (TaqMan). J Virol Methods 2004;120:229–237. 8. Allard A, Rosina G, Per J, Goran W: Polymerase chain reaction for detection of adenoviruses in stool samples. J Clin Microbiol 1990;12...

... assay; ELISA), HDV antibody (ELISA) and HDV RNA by polymerase chain reaction (PCR). The HBV genotype was determined by nested PCR using type-specific primers. The first-round PCR products from two patients were sequenced, followed by an investigation of nucleotide homology. Results: Viruses from all...

... using a monochromosomal panel in our previous study. In order to identify additional families, we examined genomic DNA derived from cancer cell lines (A549, AZ521, OVCAR3, RT4) using the PCR approach. Five env fragments of a HERV-W family were newly identified and analyzed. They showed a high degree...

... with nested PCR using primers of the S genome region, only 6 (3.3%) were HBV DNA-positive. Anti-HBc-IgM antibody could be detected in 3 (1.6 %) of the tested samples using the IMx CORE-M. With the more sensitive VIDAS HBc IgM specific IgM antibody was detected in 15 (8.5%) of 177 samples at concentrations...

... School of Medicine, Tokyo, Japan Key Words TT virus W TTV DNA W Hepatocellular carcinoma W Liver histology W Irregular regeneration, hepatocyte W PCR Abstract We tested the sera of patients with non-A, non-B, non-C, non-G (non-A to -G) hepatocellular carcinoma (HCC) for the presence of TT virus (TTV) DNA...

... of cytomegalovirus (CMV) infection in transplant recipients, we compared immediate early 1 (IE1) and late pp67 mRNA detection by NASBA with the antigenemia assay, PCR and viral culture in 72 renal transplant (RTx) recipients and with antigenemia and serology in 25 liver transplant (LTx) recipients. Antigenemia...

... intrauterine virus transmission. In AF, the virus can be detected by culture and/or PCR. Both procedures differentiate uninfected from infected fetuses, but cannot predict fetal outcome. The determination of the viral load in AF carried out by quantitative PCR is more promising and could represent an important...

... or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements. PCR Quantitation Kinetics Diagnosis Part II. Diagnostics...

... of unknown etiology and the TTV genotypes which are detectable by PCR with primers deduced from the N22 region (genotype 1) in the open reading frame 1 encoding the capsid protein. It would be important to select the primers for specific detection of the TTV genotypes associated with clinical diseases...

... replication in quiescent fibroblasts. Cytomegalovirus Dihydrofolate reductase Methotrexate PCR Quiescent cells Original Paper Intervirology 1999;42:30 36 Received: October 6, 1998 Accepted after revision: January 28, 1999 Human Cytomegalovirus Stimulates Cellular Dihydrofolate Reductase Activity...