... asymptomatic, necessitating the development of improved high-throughput screening methods to overcome the limitations of molecular diagnostics or enzyme-linked immunosorbent assays (ELISAs). Objective: In this study, two novel monoclonal antibodies (mAbs) targeting envelope 1 (E1) of CHIKV were developed...

...Jiyue Wen; Weizhuo Lu; Zhenzhen Liu; Jihong Meng Aims: To confirm the different characteristics of genotype-specific and common neutralizing epitopes of hepatitis E virus (HEV). Methods: A competitive binding assay was established with known genotype-common neutralizing monoclonal antibodies (mAbs...

...: Cell-expressing T antigens, immunoprecipitation, and immunoblot were used to determine the accessibility of amino acids. Conclusion: Full-length T-antigen and N-terminal fragments containing F98A were immunoprecipitated by monoclonal antibody PAb902, which recognizes a conformation-dependent epitope...

...Ndiémé Ndongo; Subjini Selliah; Pascale Berthillon; Valérie-Ann Raymond; Christian Trépo; Marc Bilodeau; Marie-Anne Petit Objective: To determine whether liver-derived hepatitis C RNA-containing particles express the E1E2 discontinuous antigenic determinant defined by unique monoclonal antibody...

...André D. Mbida; Odette G. Gaudin; Odile Sabido; Bruno Pozzetto; Jean-Claude Le Bihan Cell lines of primate origin carry membrane receptors which are specific for echoviruses (EV). The present report describes isolation and characterization of a monoclonal antibody (Mab 143) reacting...

...Tatsuji Hataya; Alice Kazuko Inoue; Kazusato Ohshima; Eishiro Shikata An isolate of potato virus Y, named PVY-36, reacted with polyclonal antibody against PVY-0 (an ordinary strain), but not with any of eight monoclonal antibodies (MAbs) specific to PVY-0 or with two MAbs specific to PVY-T...

...Ching-Hwa Ann Tsai; Marshall V. Williams; Ronald Glaser An Epstein-Barr virus (EBV) specific monoclonal antibody (MAb), designated 55H3, was produced after immunizing BALB/c mice with 12–0-tetradecanoylphorbol-13-acetate (TPA) activated B95–8 cells. It was possible to demonstrate that this MAb...

...Robert N. Lausch; Herman Staats; Joseph F. Metcalf; John E. Oakes The immunotherapeutic potential of a monoclonal antibody specific for glycoprotein D of herpes simplex virus was evaluated in a murine ocular infection model. Passive transfer of antibody at microgram concentrations was able...

...G.E Yousef; I.N Brown; J.F Mowbray This paper describes the derivation and characterization of monoclonal antibodies reactive with the group-specific epitopes of the VP1 peptide shared by enteroviruses. When tested against a wide range of the prototype strains of Coxsackie viruses A and B...

... paper we describe the derivation of monoclonal antibodies against epitopes of the VPI peptide which are shared by all of the enteroviruses tested to date, with the exception of hepatitis A virus. This study describes the application of one of these monoclonal antibodies in several research...

... the detection in 6M2 cells of rat-specific transformation-associated proteins (TAPs) using a monoclonal antibody (MC). In this study, we used MC to investigate further the expression of TAPs at different temperatures and the time course of turn-on and shut-off of TAPs upon temperature shifting. It was found...

... 2008 Epstein-Barr virus Monoclonal antibody Early antigen Protein kinase Oncogene © 1990 S. Karger AG, Basel 1990 Copyright / Drug Dosage / Disclaimer Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any...

...Ranajit Pal; Fulvia di Marzo Veronese; B.C. Nair; Rukhsana Rahman; George Hoke; Steven W. Mumbauer; M.G. Sarngadharan The major neutralizing epitope on the external glycoprotein of HIV-1 was studied with an envelope-specific monoclonal antibody and with a human serum positive for antibodies to HIV...

...Naoyuki Nishimura; Shingo Kato; Hiromichi Ishikawa; Toshiya Takano Mouse monoclonal antibodies were generated for three 20- to 23-mer synthetic peptides whose amino acid sequences were highly conserved in the pol or env regions of 14 mammalian and two avian retroviruses. By Western-blot analysis...

... by western blotting or by enzyme-linked immunosorbent assay experiments with three different lots of polyclonal R-MLV gp69/71 sera. However, it was detected when a specific monoclonal antibody, R47, was used in western blotting or immuno-dot-blotting experiments with the two viruses. R47 maps to a central 14...

...J.A. Aebig; R.L. Jordan; R.H. Lawson; H.T. Hsu A monoclonal antibody reacting with prunus necrotic ringspot ilarvirus was tested in immunochemical studies, neutralization of infectivity assays, and by immuno-electron microscopy. The antibody was able to detect the 27,000 Mr coat protein of prunus...

...P. Giraudon; Ch. Gerald; T.F. Wild Monoclonal antibodies were used to study measles virus (Hallé strain) infections. The distribution of virus antigens in acutely and persistently infected cells was examined by immunofluorescence, and the synthesis of the virus polypeptides in acutely infected...

...M.H. van Roosmalen; J.J. de Jong; W. Haenen; T. Jacobs; F. Couwenberg; G.J.C.M. Ahlers-de Boer; J.A. Hellings The design of a new HBsAg screening assay, the Hepanostika HBsAg Ultra is based on the use of monoclonal antibodies raised against native wild-type HBsAg and reactive with HBsAg in which...

... cells are susceptible to such microorganisms. To monitor the transmission of PERV, highly sensitive and specific immunoassays must be developed for clinical surveillance. This report describes the production, preliminary characterization and application of a monoclonal antibody (mAb) against...

...Esther Furrer; Oliver Planz; Lothar Stitz Borna disease virus-infected Vero cells express on their surface the major viral glycoprotein which mediates cell fusion after low pH treatment. This fusion event can be inhibited by monoclonal antibodies (mAbs) generated from chronically BDV-infected rats...