... polymerase transient expression system was employed to express the wild-type or mutated fusion (F) proteins. Three different types of membrane fusion assays were performed to analyze the fusogenic activity, fluorescence-activated cell sorting (FACS) analysis was executed to examine the cell surface...

... Membrane fusion Hemagglutinin-neuraminidase-fusion protein complexes Newcastle disease virus (NDV) belongs to the family Paramyxoviridae, a group of enveloped viruses with nonsegmented, negative-sense, single-stranded RNA genome [ 1 ]. It causes a series of severe respiratory and neurological...

...Bing Wu; Xiaoli Liu; Zhiyu Wang Objectives: To explore the effects of glycosylation in glycoprotein on membrane fusion in rubella virus strain JR23. Methods: The N-linked glycosylation sites in glycoproteins were mutated individually or in combination. Total expression and cell surface expression...