... were analyzed by immunofluorescence (see below), using as antigen Lausannevirus particles grown in amoebae and purified by ultracentrifugation (see below). Statistical analyses were performed using R [ 7 ]. The genome of Lausannevirus is 346,754 bp long and contains 450 genes. It exhibits...

... blot (WB) assay. This study evaluated an HIV immunofluorescent antibody (IFA) test relative to WB assay for use in confirming EIA designated HIV-1 antibody-positive sera. Specimens from seroconversion and CDC panels as well as clinical specimens obtained for routine EIA HIV-1 antibody screening were...

...Frans Hilgers; Jo Hilgers An indirect immunofluorescence (IF)1 test on fixed cells with Evans’ blue counterstain is described for all four human herpesviruses, i.e., herpes simplex virus type 1 and type 2 (HSV-1 and HSV-2), varicella-zoster virus (VZV), cytomegalovirus (CMV) and Epstein-Barr virus...

...Lars Hortling; Jukka Suni; Antti Vaheri An immunofluorescence technique was developed for the major group-specific ( gs ) p27 antigen of avian type C viruses. The localization of this antigen virus-infected in chick embryo fibroblasts was perinuclear, intracytoplasmic and at the cell surface...

... immunofluorescence with counter staining. During the period shortly after infection, the virus was localized in distal parts of the lateral lobules of the salivary glands, whereas during the time of maximum multiplication it was concentrated in the anterior parts. Detection of the virus in the salivary glands...

... simplex virus Survivor cells Neuronal cells Dot blot hybridization Immunofluorescence Original Papers Intervirology 23: 181-189 (1985) Herpes Simplex Virus Type 1 and Neuronal Cells - a Special Cell-Virus Interaction V. Veerisetty3, G.A. Gentrya. I.K. Veerisetty J.P. Kappc, J. Staczek3 Departments...

...Michael B.A. Oldstone; Shudo Yamazaki; Akira Niwa; Abner Louis Notkins The percentage of cells infected with lactic dehydrogenase virus (LDV) in primary mouse embryo cells was studied by immunofluorescent and infectious center assays. Data from both techniques indicated that only a small percentage...

...Bert L. Murphy; John M. Peterson; James W. Ebert; Kenneth R. Berquist; James E. Maynard; Robert H. Purcell Three chimpanzee chronic carriers of hepatitis B surface antigen (HBS Ag) were examined by immunofluorescent techniques to determine the localization of HBS Ag and hepatitis B core antigen...

...S.R. Pal; Gy. Szücs; J.L. Melnick During 1981–1982, epidemics of acute hemorrhagic conjunctivitis (AHC) occurred in and around Chandigarh, India. Conjunctival swabs were taken from AHC cases and investigated by the indirect immunofluorescence method both in Chandigarh and in Houston. By using...

...P. Giraudon; Ch. Gerald; T.F. Wild Monoclonal antibodies were used to study measles virus (Hallé strain) infections. The distribution of virus antigens in acutely and persistently infected cells was examined by immunofluorescence, and the synthesis of the virus polypeptides in acutely infected...

... cultures and is applicable to various virus-host cell systems. 20 10 1982 24 7 2008 Immunofluorescence p30 antigen Xenotropic Host range © 1983 S. Karger AG, Basel 1983 Copyright / Drug Dosage / Disclaimer Copyright: All rights reserved. No part of this publication may...

...Guadalupe Carballal; Patricio M. Cossio; Adriana Rabinovich; José Oubiña; Roberto M. Arana A BHK/21 cell line persistently infected by an arenavirus is described. During four consecutive passages, 30–45% of the cells showed granular cytoplasmic antigen by indirect immunofluorescence, employing both...

... in the cells by indirect immunofluorescence. Application of this technique to patients with Argentine hemorrhagic fever may lead to the quickest method for the virologic diagnosis of this disease. Short Communication Intervirology S: 360-363 (1977) A Rapid Method for Detecting Junin Virus Viremia in the Guinea...

... of the group. These relationships are particularly evident in evaluations of human convalescent sera. New World arenaviruses cross-react strongly with each other in most instances but only weakly with Old World arenaviruses. Arenaviruses Immunofluorescence Cross-reactions 25 05 1977 23 7...

...-IgM antibodies were detected in sera of patients with IM and CAEBV but not in sera of healthy controls. In sera of patients with CAEBV, the titers of IgG antibodies to ZEBRA correlated with the antibody titers to early antigens obtained with an immunofluorescence assay, but not to EBV nuclear antigens...