.... Higher the electroporation voltages inflict greater pain, and this limits the circumstances in which the technique can be used clinically. The voltages generally used for electroporation in animals range from 100 to 1,200 V/cm. We studied the effect of DNA vaccination when electroporation was performed...

...Robert G. Whalen Occasionally, a major change in vaccine methodology comes along. Such would appear to be the case with the advent of DNA-mediated immunization, colloquially known as DNA vaccines. This represents a radical new way to deliver antigens; it involves the direct introduction...

...: September 28, 2005 Accepted after revision: February 14, 2006 Published online: June 29, 2006 Enhanced Induction of SARS-CoV Nucleocapsid Protein-Specific Immune Response Using DNA Vaccination followed by Adenovirus Boosting in BALB/c Mice Ma Chunlinga Yao Kuna Xu Jiana Qin Jianb Sun Huac Zhu Minshengd...

...Andreas Henke; Nadine Rohland; Roland Zell; Peter Wutzler A promising approach to protect susceptible individuals against severe diseases is the inoculation of plasmids. Such DNA vaccines against influenza virus infections were quite efficient in different animal models; but still this procedure...

...-and-mouth disease virus (FMDV) DNA vaccine could increase the protective immune response. Mice were vaccinated twice with either FMDV DNA vaccine plus the Isatis indigotica extract or DNA vaccine alone. Compared with the group of DNA vaccine alone, in the group that received DNA vaccine plus the Isatis...

...Kyung Koo Kang; Seul Min Choi; Jae Hoon Choi; Dong Sup Lee; Chae Young Kim; Byung Ok Ahn; Byung Moon Kim; Won Bae Kim AIDS is one of the greatest infectious disease threats to human health despite the extensive efforts made since the discovery of HIV in 1983. The use of plasmid DNA vaccination...

.... HIV Synthetic genes DNA vaccine T cell immunogenicity Intervirology 2002;45:275 286 DOI: 10.1159/000067919 Impact of Codon Usage Modification on T Cell Immunogenicity and Longevity of HIV-1 Gag-Specific DNA Vaccines Alexandra Bojak Jens Wild Ludwig Deml Ralf Wagner Institute of Medical...

... of DEN or JE virus, recombinant poxviruses, recombinant baculoviruses, protein expression in Escherichia coli , and naked DNA vaccines. This report summarizes some of the recent developments in DEN and JE vaccinology, particularly vaccine strategies that involve live attenuated viruses, engineered...

...Reinhold Schirmbeck; Jörg Reimann Using different DNA vaccination techniques, we studied the IgG1/IgG2a antibody and MHC-I-restricted cytotoxic T lymphocyte (CTL) responses to the hepatitis B surface antigen (HBsAg) in mice. A single intramuscular injection of 100 µg HBsAg-encoding pCI/S plasmid...

... or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements. HIV-1 Protection DNA vaccine Reverse transcriptase Mouse model...

... the frequency of integration of plasmid DNA vaccines into host cellular DNA, including DNA sequences within the plasmid, the expressed gene product (antigen), the formulation, delivery method, route of administration, and the type of cells exposed to the plasmid. In this report, we examined the tissue...

... responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements. NK cells HIV-1 DNA vaccine Intervirology 2000;43:294 301 Human Natural Killer Cells in Asymptomatic Human Immunodeficiency Virus-1 Infection...

...J. Pavlovic; J. Schultz; H.P. Hefti; T. Schuh; K. Mölling For the development of effective conventional vaccines or DNA vaccines against viruses, the availability of suitable animal models is an essential prerequisite. For many recently emerging zoonotic viruses, suitable animal models are still...

... to induce cellular as well as humoral immunity. In this study we investigate the mechanism of protection after intramuscular vaccination of C57Bl/6 mice with a DNA vaccine encoding HA of influenza A/PR/8/34. After a single injection, only a small percentage of mice survive the lethal challenge...

...Dan H. Barouch; Norman L. Letvin Control of the worldwide AIDS epidemic will only be achieved with a safe and effective prophylactic HIV-1 vaccine. DNA vaccination has recently emerged as a promising vaccine modality that can elicit both humoral and cellular immune responses. HIV-1- and SIV...

...Brian J. Ledwith; Sujata Manam; Philip J. Troilo; Amy B. Barnum; Cindy J. Pauley; Thomas G. Griffiths II; Laural B. Harper; Carolann M. Beare; Walter J. Bagdon; Warren W. Nichols The primary safety concern for DNA vaccines is their potential to integrate into the host cell genome. We describe...