Objective: The research of adjuvants is a hot topic in vaccinology. In this paper, we were seeking a concise method to produce a stable and effective emulsion adjuvant. Methods: A novel emulsion [Well Adjuvant Formulation 3 (WAF3)] was produced by adding additional glycerol and using a timely cooling process. Surface morphology analysis of the novel emulsion was performed by atomic force microscopy. Animal experimentation was used to evaluate the efficacy of the novel adjuvant. Results: Surface morphology analysis of the novel emulsion showed a homogeneous distribution of the antigen protein. We also confirmed the morphological changes of the WAF3-carrying antigen. The WAF3-adjuvanted vaccine engendered higher antibody responses 128-fold compared to that of a naked antigen. Furthermore, in the presence of the WAF3 adjuvant, the influenza virus antigen was able to reduce the titer disparities of IgG2a and IgG1. The WAF3-adjuvanted influenza vaccine was able to provide full protection and alleviation of infective symptoms as other clinically adjuvanted vaccines. Conclusion: The WAF3 adjuvant is a promising candidate to be further investigated in the development of emulsion adjuvants.

Keywords:
Oil emulsion, APR8 vaccine, MF59, WAF3, Adjuvant
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© 2009 S. Karger AG, Basel
2009
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