It has been reported that blood basophils comprise two populations of different densities. In this study, we compared the responses of two populations of basophils (band-1 and 2 basophils) to several secretagogues and anti-allergic agens. Twelve patients with bronchial asthma, 3 patients with Japanese ceder pollinosis and 6 healthy subjects were included in this study. Band-2 basophils collected from the interface between Percoll of density 1.078 and 1.068 g/ml released histamine better in response to anti-IgE than band-1 basophils taken from the interface between plasma and Percoll 1.068 (band 1 vs. band 2, 13.2 ± 2.7 vs. 21.4 ± 3.6% at 1:105, p < 0.01; 47.1 ± 5.0 vs. 57.7 ± 6.1% at 1:104, p < 0.01). Band-2 basophils also responded better to formylmethionyl-leucyl-phenylalanine than band-1 basophils (34.4 ± 4.9 vs. 43.2 ± 5.6% at 10–6 M, p < 0.01; 35.6 ± 5.0 vs. 45.2 ± 5.7; at 10–5 M, p < 0.01). In contrast, band-1 basophils were much more sensitive to the calcium ionophore A23187 stimulation than band-2 basophils (13.0 ± 3.7 vs. 6.3 ± 1.8% at 0.05 µg/ml, p < 0.05; 52.5 ± 4.3 vs. 33.8 ± 3.9% at 0.1 µg/ml, p < 0.01; 71.3 ± 3.3 vs. 51.5 ± 4.2% at 0.2 µg/ml, p < 0.01). Similarly, band-1 basophils were more responsive to 12-O-tetradecanoylphorbol-13-acetate than band-2 basophils (67.8 ± 6.3 vs. 53.1 ± 8.4% at 1.0 ng/ml, p < 0.01; 63.9 ± 7.4 vs. 45.2 ± 7.2% at 10 ng/ml, p < 0.01). Band-1 and 2 basophils had similar susceptibility to inhibition by fenoterol, theophylline and disodium cromoglycate of anti-IgE-induced histamine release. These results indicate that human basophils were separated into two subpopulations, low and high density basophils with different sensitivity to secretagogues.

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© 1989 S. Karger AG, Basel
1989
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