The allergenic potencies of samples of two commercial Alternaria tenuis extracts stored under various conditions were compared at 3 months intervals over a period of 1 year. Aliquots of the extracts at 5.12 mg/ml were maintained in: (a) saline; (b) 50% glycerol-saline mixture; (c) 0.4% phenol-saline; (d) 0.4% phenol-saline containing 0.03% human serum albumin, and (e) samples were also kept in lyophilized form. The samples were stored at ––20, 4, 24, 37 and 56°C. Their potency was measured by radioallergosorbent inhibition assay and by mouse IgE passive cutaneous anaphylaxis (PCA) tests. The lyophilized extracts fully maintained their activity at all temperatures for 12 months. Aqueous extracts with or without preservatives also maintained their potency if stored at ––20,4 and 24°C, but showed a significant loss of PCA activity at 56°C, after 3 months. A deleterious effect of phenol was observed in one of the extracts stored at ––20°C for 3 months, by PCA tests. The use of preservatives to retain potency over a period of 12 months is therefore unnecessary and may be deleterious. The results indicate that, while storage of A. tenuis in a lyophilized form is best at all temperatures, aqueous extracts are also remarkedly stable when stored at ––20, 4 and 24°C, over a 12-month period.

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© 1987 S. Karger AG, Basel
1987
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