A ubiquitous tissue antigen (UTA), associated with chronic renal diseases was partially purified and characterized. The antigen was extracted by solubihzation in 0.5% sodium deoxycholate (DOC) of various organs of human and animal origin. UTA was soluble in ammonium sulfate at 50% concentration and precipitable in 71% ethanol. Considerable purification of UTA was achieved by fractional ammunium sulfate precipitation, zone electrophoresis and gel filtration. Gel filtration on Sephadex G-200 and velocity gradient centrifugation through 10–40% sucrose gradients suggested that the main antigenic component is a macromolecule, although aggregability of DOC-extracted proteins upon removal of the solubilizer prevented more accurate determinations. UTA appeared to be a heat-stable glycoprotein which did not contain lipids detectable by Sudan black B staining. The yield of purest UTA preparations, which did not contain components of homologous serum as tested by double diffusion gel precipitation or immunoelectrophoresis, was 0.1–0.2 mg/100 g of starting tissue. Immunization of rabbits with homologous UTA-containing preparations resulted in anti-UTA antibody formation.

This content is only available via PDF.
Copyright / Drug Dosage / Disclaimer
Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.
You do not currently have access to this content.